白玉蔗组织培养研究

目的]以广西贵港市白玉蔗为材料,探讨影响白玉蔗离体培养的主要因素,建立其快速再生繁殖体系.方法]以白玉蔗尾梢心叶为外植体,诱导愈伤组织并进行继代培养后,分别进行不同培养基和激素组合对白玉蔗愈伤组织芽分化培养、不定芽增殖和生根壮苗等影响试验.结果]白玉蔗心叶切片供诱导愈伤组织是方便快捷的有效方法.心叶切片接人培养基(MS+2,4-D 3 mg/L,琼脂5g/L,糖30 g/L)30 d后,外植体迅速膨胀,边缘出现颗粒状、淡黄或黄色、质地均一的胚性愈伤组织或糊状的愈伤组织.诱导白玉蔗芽分化以培养基MS+6-BA 1.0 mg/L+ NAA0.2 mg/L的效果最佳;培养不定芽增殖以MS+6-BA 3.0 mg/L+KT 0.5 mg/L+NAA 0.6 mg/L+糖30.0 g/L培养基的效果最佳.PVP和活性炭对防止组培苗褐变的效果有差异,1%活性炭能有效抑制白玉蔗增殖培养基中丛生芽的褐化.在生根壮苗培养中,最优培养基为MS+ NAA 0.6 mg/L+糖50.0 g/L+活性炭0.1%.结论]通过正交试验设计,建立了白玉蔗的再生繁殖体系,为加快白玉蔗繁殖速度、提高繁殖系数、节约种茎提供有效途径.

Tissue culture of Baiyu chewing cane

【Objective】Using the Baiyu chewing cane collected from Guigang City， Guangxi as testing material， the main factors impacting Baiyu chewing cane in vitro culture was explored in order to establish the rapid regeneration propagation system. 【Method】After using the tissues from Baiyu chewing cane’s slightly-tailing leaves as explant callus for bud differentiation culture of callus， adventitious bud proliferation， and plantlet browning prevention and rooting induction were conducted using different culture medium and hormone combinations. 【Result】Baiyu chewing cane leaf slicing for callus induction was a convenient and effective method. After the whorl sections were connected with the culture medium （MS+2，4-D 3 mg/L， agar 5 g/L， sugar 30 g/L） for 30 d， explants rapidly expanded， the edges became granular， light yellow or yellow， and the embryogenic callus or paste callus displayed uniformed quality. The optimum culture medium for buds differentiation induction in Baiyu chewing cane was MS+6-BA 1.0 mg/L+ NAA0.2 mg/L. For the proliferation of adventitious buds， the optimum effect resulted from the MS+6-BA 3.0 mg/L+KT 0.5 mg/L+NAA 0.6 mg/L+ sugar 30.0 g/L treatment. To prevent browning effects， the PVP and activated carbon tissue culture seedlings had different effects； the 1% activated carbon could effectively inhibit the proliferation of adventitious shoots browning in Baiyu chewing cane culture medium. In the rooting culturing process， the optimal culturing medium was MS+ NAA 0.6 mg/L+ sugar 50.0 g/L + activated carbon 0.1%. 【Conclusion】By the orthogonal design test， the regeneration system of Baiyu chewing cane breeding was established in order to speed up the breeding rate of Baiyu chewing cane， improve propagation coefficient， and provide an effective way for capital-saving.