超声提取永思小檗盐酸小檗碱工艺的优化

【目的】优选出永思小檗盐酸小檗碱的最佳超声提取工艺条件，为盐酸小檗碱在医药工业上的研发应用奠定基础。【方法】以乙醇为溶媒，采用超声提取法提取永思小檗盐酸小檗碱，并通过单因素试验和正交设计L9（34）考察乙醇浓度、提取时间、提取温度、溶媒倍数对永思小檗盐酸小檗碱提取效果的影响。【结果】综合考虑成本及提取效率，永思小檗盐酸小檗碱最佳超声提取工艺为：以150倍45%乙醇作溶媒，60℃超声提取一次，时间为40 min。【结论】在最佳工艺条件下，超声提取永思小檗盐酸小檗碱的平均含量达11.9633 mg/g，进一步证实以乙醇为溶媒、采用超声提取法提取小檗碱的可行性。

Optimization of technology conditions for ultrasonic extraction of berberine hydrochloride from Berberis tsienii Ying

Objective]Optimal technology for extracting berberine hydrochloride from Berberis tsienii Ying was studied to accelerate researches and development on berberine hydrochloride in medical industry.Method]Berberine hydrochloride was extracted using ethanol solvent by ultrasonic extraction method.Single factor experiment and L9(34) orthogonal design were used to observe effects of ethanol concentration,extraction time,extraction temperature and solvents on berberine hydrochloride extraction.Result]When 150 times ethanol(45%) was used as solvent to extract berberine hydrochloride at 60℃ for 40 min,extraction efficiency was recorded as highest and the cost was the lowest.Conclusion]Under the best technology conditions,the average content of berberine hydrochloride attained 11.9633 mg/g concentration indicating that it’s viable to use ethanol and ultrasonic method for berberine hydrochloride extraction.