辣椒疫病罹病植株根际土壤细菌群落多样性分析

目的]分析辣椒疫病罹病植株根际土壤细菌群落多样性,为辣椒疫病防控提供理论依据.方法]采集湖南长沙、吉首及永州地区辣椒疫病罹病植株根际土壤,运用IlluminaMiSeq高通量测序技术对土壤样品基因组DNA的16S rDNA-V4区进行测序分析,明确不同地区罹病辣椒植株根际土壤细菌群落多样性.结果]3个地区罹病辣椒根际土壤的细菌OTU数量存在差异,独有OTU占总体比例各有差异,其中永州地区占其总数的30.18%、吉首地区占其总数的41.83%、长沙地区占其总数的24.07%,3个地区共有OTU占所有检测到OTU的14.95%.α多样性指标均表现为长沙地区最低,吉首地区最高.在门水平上,3个地区的罹病辣椒根际土壤微生物组成相似,优势菌门为Acidobacteria(酸杆菌门)、Proteobacteria(变形菌门)、Actinobacteria(放线菌门)和Chloroflexi(绿弯菌门),但3个地区在相对丰度上表现一定差异.结论]湖南永州、吉首和长沙地区辣椒疫病罹病植株根际土壤细菌多样性存在差异,以吉首最高、长沙最低;3个地区的罹病辣椒根际土壤微生物组成相似,但相对丰度存在差异.

Comparison on bacterial community diversity in rhizosphere soil of peppers with phytophthora blight

Objective]In the present study, bacterial community diversity in rhizosphere soil of peppers with phytoph-thora blight was studied to provide reference for control of pepper phytophthora blight. Method]Rhizosphere soil of peppers dying from phytophthora blight was collected from Changsha, Jishou and Yongzhou in Hunan. 16S rDNA-V4 regions of sample genome DNA were sequenced by IlluminaMiSeq high-throughput sequencing to analyze bacterial community diver-sity in rhizosphere soil of peppers with phytophthora blight in different areas. Result]Bacteria OTU number and propor-tions of unique OTU of rhizosphere soil of peppers with phytophthora blight of three areas were different. The proportion of Yongzhou accounted for 30.18% of the total, and that of Jishou accounted for 41.83% of the total and that of Changsha accounted for 24.07% of the total. Shared OTU of the three areas accounted for 14.95% of all detected OTU. Alpha diversity index were the lowest in Changsha and the highest in Jishou. At phylum level, the microbial composition was similar in rhi-zosphere soil of peppers with phytophthora blight of three areas, the dominant bacteria were Acidobacteria, Proteobacteria, Actinobacteria and Chloroflexi, but relative abundance showed some differences. Conclusion]There is difference in bac-terial community diversity in rhizosphere soil of peppers dying from phytophthora blight in Yongzhou, Jishou and Chang-sha, the diversity of Jishou is the highest while that of Changsha is the lowest. The microbial composition is similar in rhizo-sphere soil of peppers with phytophthora blight of the three areas, but the relative abundance is different.