新型黄病毒在种鹅中垂直传播的研究

目的]证实新型黄病毒在种鹅中是否存在垂直传播,为控制该病提供可靠的流行病学依据.方法]采用巢式RT-PCR和病毒分离技术,对患病种鹅病料、不同孵化阶段死胚、出雏蛋壳内部冲洗液、弱雏样品进行黄病毒分离及基因检测.结果]在患病种鹅、孵化过程中死亡鹅胚尿囊液、出雏蛋壳内壁洗液和雏鹅中均能检测和分离到黄病毒,其中患病种鹅病料的黄病毒阳性率为100.0%,孵化死亡鹅胚黄病毒阳性率为39.6%,出雏蛋壳内部冲洗液黄病毒阳性率为45.0%;1日龄和15日龄弱雏黄病毒阳性率为80.0%.结论]黄病毒可以通过鹅胚传到下一代,即新型黄病毒在种鹅中存在垂直传播的现象.

Vertical transmission of new type flavivirus in egg-laying goose

Objective]The present experiment was performed to investigate the vertical transmission of a new type flavivirus in egg-laying goose in order to provide essential epidemiological data for controlling flavivirus infection. Method]The isolation of flavivirus and detection of its gene in different stages dead embryos, post-hatching eggshell wall and weak offspring samples of sick egg-laying goose were carried out using nest RT-PCR. Result]Flavivirus was isolated and detected from all the above-mentioned samples, and the rate of flavivirus occurrence was recorded as 100.0, 39.6 and 45.0% in sick goose, dead embryos and post-hatching egg shell wall, respectively. The positive rate of flavivirus was 80.0% in weak offspring at 1 as well as at 15 days after hatching. Conclusion]The flavivirus can be passed on to the next generation via embryo, i.e., the new type of flavivirus transmits vertically in egg-laying goose.