广西主栽葡萄品种果实白藜芦醇及其糖苷HPLC定量分析

[目的]广西湿热气候条件下生长成熟的葡萄中白藜芦醇及白藜芦醇苷的含量,为今后进一步研究南方地区鲜食与酿酒葡萄奠定基础.[方法]以广西湿热地区主栽葡萄品种巨峰、夏黑、Fry、Carlos和Nobel为材料,使用高效液相色谱法( HPLC)定量分析其果实白藜芦醇和白藜芦醇苷含量.[结果]在5个品种中,巨峰葡萄果实中的白藜芦醇苷含量最高,达到15.621 mg/L,其次是夏黑,为12.062 mg/L;白藜芦醇含量最高是Nobel,为0.276 mg/L,其次是巨峰,为0.206 mg/L;巨峰葡萄具有较高的白藜芦醇及其糖苷含量,其总含量达到15.827 mg/L,其次是夏黑葡萄,其总含量达到12.048 mg/L.[结论]巨峰葡萄在广西湿热环境中能产生较多的白藜芦醇.

Determination of trans-resveratrol and its glucoside content in pulp of five grape varieties in Guangxi using HPLC

[Objective]The present experiment was conducted to study the resveratrol and polydatin content in peel of grapes grown under muggy weather conditions of Guangxi.[Method]Five grape varieties viz.,Kyoho,Xiahei,Fry,Carlos and Nobel,which were cultivated under muggy weather conditions of Guangxi,were used to determine the resveratrol and polydatin content in peels using HPLC.[Result]The result showed that Kyoho grape recorded 15.621 mg/L polydatin,which was higher than other grape varieties,followed by Xiahei(12.062 mg/L).The peel of Nobel showed the highest(0.276 mg/L) resveratrol content,followed by Kyoho(0.206 mg/L).The total content of resveratrol and polydatin in Kyoho reached 15.827 mg/L,which was the highest among all the varieties,followed by Xiahei,it was 12.048 mg/L.[Conclusion]The grape variety Kyoho can produce more resveratrol when cultivated under muggy weather conditions of Guangxi.