加味大柴胡汤对阻塞性黄疸老年大鼠TRPC1表达的影响

目的 探讨加味大柴胡汤在临床上辅助治疗老年阻塞性黄疸的理论机制。方法 通过结扎胆总管的外科手术方法建立阻塞性黄疸老年大鼠模型，实验分为假手术组、模型组、加味大柴胡汤组，予以相应干预，分3、7、10 d共3个时间段，每组10只SPF级老年SD大鼠，在各时间段取老年大鼠肝组织及心脏血，检测AST、ALT及TBA；采用钙离子试剂盒检测老年鼠肝组织匀浆钙离子浓度；采用Western Blot法检测老年鼠肝组织TRPC1蛋白的表达，采用Tunel法观察肝细胞凋亡情况。结果 造模成功后各时间点模型组、加味大柴胡汤组与假手术组比较，ALT、AST、TBA和Ca²⁺浓度均升高，且模型组上升更明显（P<0.01），模型组、加味大柴胡汤组的TRPC1的蛋白含量也均高于假手术组（P<0.01），且加味大柴胡汤组肝细胞的凋亡率低于模型组（P<0.01）。结论 梗阻性黄疸老年大鼠TRPC1表达明显升高，加味大柴胡汤下调TRPC1的表达，可能是其降低钙超载，减少肝细胞凋亡的机制之一。

Effects of Jiawei Dachaihu Decoction on TRPC1 Expression in Aged Rats with Obstructive Jaundice

Objective To investigate the theoretical mechanism of Jiawei Dachaihu Decoction in the treatment of obstructive jaundice in the aged rats. Methods The old rat model of obstructive jaundice was established by the surgical method of ligating the common bile duct. The experiment was divided into a sham operation group, a model group and a Jiawei Dachaihu Decoction group. Corresponding interventions were given for 3 time periods of 3, 7 and 10 d, with 10 SPF aged SD rats in each group. The liver tissue and heart blood of aged rats at various time points were taken to detect AST, ALT and TBA; Calcium ion kit was used to detect the calcium ion concentration in liver tissue of aged rats. The expression of TRPC1 protein in liver tissue of aged rats was detected by Western Blot. The apoptosis of hepatocytes was observed by Tunel method. Results After successful modeling, the ALT, AST, TBA and Ca²⁺ concentrations were increased in the model group, the Jiawei Dachaihu Decoction group and the sham operation group, and the model group increased significantly (P<0.01). The protein content of TRPC1 in the model group and the Jiawei Dachaihu Decoction group was also higher than that in the sham operation group (P<0.01), and the apoptosis rate of hepatocytes in Jiawei Dachaihu Decoction group was lower than that in model group (P<0.01). Conclusion The expression of TRPC1 is significantly increased in aged rats with obstructive jaundice. The expression of TRPC1 is down-regulated by Jiawei Dachaihu Decoction, which may be one of the mechanisms to reduce calcium overload and reduce hepatocyte apoptosis.