基于p38 MAPK信号通路研究养阴润目丸治疗干眼兔的作用机制

目的 探讨基于p38 MAPK信号通路研究养阴润目丸治疗干眼兔的作用机制。方法 采用氢溴酸东莨菪碱皮下注射途径制备干眼兔模型，按随机数字表法分成模型组、p38抑制剂组、阳性药物（杞菊地黄丸）组和低、中、高剂量养阴润目丸组，同步设不造模的正常组，6只/组。各组按相应处理方式连续干预2周后，检测各组兔泪液分泌量、泪腺病理形态、泪腺组织凋亡细胞的表达及泪腺组织中p38 MAPK、Bax、Bcl-2蛋白和mRNA表达情况。结果 养阴润目丸组和阳性药物组能增加干眼兔模型泪液分泌量，抑制泪腺细胞的凋亡，下调p38 MAPK、Bax的蛋白和mRNA表达，上调Bcl-2的蛋白和mRNA表达水平，与模型组比较，差异均有统计学意义（P<0.05）。结论 养阴润目丸能够通过纠正Bax/Bcl-2失衡，改善干眼泪腺细胞凋亡，进而减轻泪腺细胞损伤，恢复泪腺细胞的代谢功能，与调控p38 MAPK的关系极为密切，这可能是其治疗干眼的重要机制之一。

Study on the Mechanism of Yangyin Runmu Pills in the Treatment of Rabbit with Dry Eye Based on p38 MAPK Signaling Pathway

Objective To explore the mechanism of Yangyin Runmu Pills in the treatment of rabbit model with dry eye based on p38 MAPK signaling pathway. Methods Dry eye rabbit models were established by subcutaneous injection of scopolamine hydrobromide. According to random number table method, they were divided into model group, p38 inhibitor group, positive medicine (Qiju Dihuang Pills) group, low, medium and high dose Yangyin Runmu Pills groups, and normal group without modeling, 6 rabbits/group. After 2 weeks of continuous intervention, lacrimal secretion volume, pathological morphology of lacrimal gland, expression of apoptotic cells in lacrimal gland tissue, as well as expressions of p38 MAPK, Bax and Bcl-2 protein and mRNA in lacrimal gland tissue were detected. Results In the Yangyin Runmu Pills groups and the positive medicine group, the lacrimal secretion volume was increased, the apoptosis of lacrimal gland cells was inhibited, the expressions of p38 MAPK, Bax protein and mRNA were down-regulated, and the expression levels of Bcl-2 protein and mRNA were up-regulated, which were significantly different from those in the model group (P<0.05). Conclusion Yangyin Runmu Pills can improve the apoptosis of dry eye lacrimal gland cells by correcting the imbalance of Bax/Bcl-2, thereby alleviating the damage of lacrimal gland cells and restoring the metabolic function of lacrimal gland cells, and it is closely related to the regulation of p38 MAPK, which may be one of the important mechanisms in the treatment of dry eye.