左归丸对去势大鼠钙转运通路相关蛋白的影响

目的 探讨左归丸去势大鼠骨密度和骨、肾组织中钙转运通路相关蛋白的影响。方法 选取48只雌性SD大鼠，随机分为正常组，假手术组，模型组，西药组，中药低、高剂量组。除假手术组和正常组，其余组切除大鼠卵巢，造模成功后3个月进行各组干预。3个月后处死大鼠，检测大鼠骨密度（BMD）；Western blotting法检测钙转运通路（肾组织）蛋白表达；免疫组化检测去势大鼠骨组织中新型上皮钙通道5（TRPV5）蛋白的表达。结果 与模型组相比，左归丸高剂量组与西药组大鼠骨密度均有改善（P<0.05）；与假手术组比较，模型组各蛋白表达均有不同程度下降（P<0.01，P<0.05）；尼尔雌醇和左归丸均能不同程度上调去势大鼠肾组织中TRPV5蛋白、钠钙交换器（NCX1）蛋白、钙结合蛋白-D28K（CaBP-D28K）和细胞膜钙汞（PMCA1b）蛋白表达，差异均具有统计学意义（P<0.05，P<0.01）；免疫组化显示模型组较假手术组的TRPV5蛋白表达率增加（P<0.05），而西药组和中药高、低剂量组较模型组蛋白表达率均下降（P<0.05）。结论 左归丸可通过上调肾钙转运通路中相关蛋白的表达和降低大鼠破骨细胞中TRPV5蛋白的表达，而发挥骨质疏松症治疗作用，肾、骨组织中钙转运过程相关蛋白可能成为骨质疏松症治疗的新方向、新靶点。

Effects of Zuogui Pills on Calcium Transport Pathway-related Proteins in Ovariectomized Rats

Objective To explore the effects of Zuogui Pills on bone mineral density (BMD) and related proteins involved in calcium transport pathway in bone and renal tissues of ovariectomized rats. Methods A total of 48 female SD rats were randomly divided a normal group, a sham operation group, a model group, a western medicine group, and a low and high dose Zuogui Pills group. Except the sham operation group and the normal group, ovaries of the rats in the other groups were removed, and the intervention of each group was carried out 3 months after the successful establishment of the model. Three months later, the rats were killed to detect the BMD; expressions of proteins involved in calcium transport pathway (renal tissue) was detected by Western blotting; and protein expression of TRPV5 in bone tissue of ovariectomized rats was detected by immunohistochemistry. Results Compared with the model group, the BMD of rats in the high dose Zuogui Pills group and western medicine group was increased (P<0.05); compared with the sham operation group, the expressions of proteins in the model group were decreased in varying degrees (P<0.01, P<0.05); Nilestriol and Zuogui Pills could increase the expressions of TRPV5 (transient receptor potential cation channel, subfamily V, member 5) protein, NCX1 (Sodium-calcium exchanger 1), CaBP-D28K (Calcium-binding protein-D28K) and PMCA1b (Plasma membrane Ca2-ATPase 1b) in the renal tissue of ovariectomized rats in different degrees, and the differences were statistically significant (P<0.05, P<0.01); immunohistochemistry showed that the expression of TRPV5 protein in the model group was increased compared with that in the sham operation group (P<0.05), while the protein expression rates in the western medicine group as well as high and low dose Zuogui Pills groups were decreased compared with that in the model group (P<0.05). Conclusion Zuogui Pills can play a therapeutic role in osteoporosis by increasing the expressions of related proteins involved in renal calcium transport pathway and decrease the expression of TRPV5 protein in rat osteoclasts. The related proteins involved in calcium transport pathway in bone and renal tissues may become a new direction and target in the treatment of osteoporosis.