参麦注射液对高血压心衰大鼠血浆代谢组学的影响

目的 研究参麦注射液对高血压心衰大鼠血浆代谢组学的影响。方法 通过8% NaCl高盐饲料喂养Dahl盐敏感性大鼠制造高血压心衰模型，成模大鼠分为模型组、参麦注射液组，并同时设置正常组，干预15 d后，采集正常组、模型组和参麦注射液组大鼠的血浆样本，运用气相色谱-质谱（GC-MS）联用技术及多元统计分析进行研究。结果 3组的代谢图谱存在显著差异。与正常组相比，模型组19种代谢物水平紊乱；经参麦注射液干预后，亮氨酸、鸟氨酸、丙氨酸、4-羟基-脯氨酸、苏氨酸、天冬酰胺、苏糖醇、丙酮酸、尿素浓度向正常方向显著回调，苏糖酸、半胱氨酸、色氨酸、甲基半胱氨酸、花生四烯酸、胆固醇、木糖醇、核糖醇、焦谷氨酸、丙二酸浓度无显著改变，此外还发现异亮氨酸浓度上升。结论 高血压心衰大鼠血浆代谢组学出现变化，参麦注射液能有效改善部分紊乱的代谢物水平，其作用机制与改善糖代谢、氨基酸代谢紊乱，调节支链氨基酸水平相关，未发现有改善脂代谢紊乱的作用。

Effects of Shenmai Injection on Plasma Metabolomics in Rats with Hypertensive Heart Failure

Objective To study the effects of Shenmai Injection on plasma metabolomics in rats with hypertensive heart failure. Methods The hypertensive heart failure model was established by continuously feeding Dahl salt-sensitive rats with 8% NaCl diet. After modeling, the rats were randomly divided into model group, Shenmai Injection group and normal group. After 15 d of intervention, plasma samples of the normal group, the model group, and the Shenmai Injection group were collected, and gas chromatography-mass spectrometry (GC-MS) coupling technique and multivariate statistical analysis were applied for the study. Results There were significant differences in metabolic patterns of the 3 groups. Compared with the normal group, there were disorders in the levels of 19 metabolites in the model group; the concentrations of leucine, ornithine, alanine, 4-hydroxy-proline, threonine, asparagine, threitol, pyruvic acid and urea intervened by Shenmai Injection were significantly back-regulated towards the normal level, and there were no significant differences in the concentrations of threonic acid, cysteine, tryptophan, methyl cysteine, arachidonic acid, cholesterol, xylitol, ribitol, pyroglutamic acid and malonic acid. In addition, it was found that the concentration of isoleucine was increased. Conclusion The changes of plasma metabolomics in heart failure rats with hypertension indicates that Shenmai Injection can effectively improve the levels of some disordered metabolites. The mechanism of Shenmai Injection is related to the improvement of disorders in glucose metabolism and amino acid metabolism, and the regulation of the levels of branched chain amino acid, but no improvement of lipid metabolism disorder is found.