| 不同生境Bt分离株晶体含量与靶标昆虫生物活性关系分析 |
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| 作者姓名： | 潘晓鸿 王飞鹏 吴小凤 孙霖瑛 林志艺 关雄 |
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| 作者单位： | 潘晓鸿,王飞鹏,吴小凤,孙霖瑛,林志艺,关雄,Pan Xiaohong,Wang Feipeng,Wu Xiaofeng,Sun Linying,Lin Zhiyi,Guan Xiong(福建农林大学生物农药与化学生物学教育部重点实验室,福州,350002);姚荣英,Yao Rongying(福建省浦城县浦城绿安生物农药有限公司,福建浦城,353400)
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| 基金项目： | 国家高技术研究发展计划(863计划)，教育部高等学校博士学科点专项科研基金，省科技创新平台建设项目，福建农林大学大学生创新性实验项目? |
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| 摘 要： | 通过采用醋酸钠-抗生素筛选法从6个不同生境中筛选得到了苏云金芽孢杆菌新菌株W14、ZY1、3346、TMQ2、B31和P91,镜检可观察到其伴孢晶体呈小菱形和不规则形状.SDS-PAGE分析结果表明6个菌株表达的晶体蛋白分子量为130kDa,对其杀虫晶体蛋白的测定,结果发现这些菌株晶体蛋白含量均不高,仅为0.20%～0.29%,而B31则无晶体含量.同时对这6个Bt菌株进行了生物测定,发现所有菌株对棉铃虫杀虫活性均较低.由此可见,特殊生境获得的Bt菌株与常规菌株晶体含量不同,且晶体含量过低是其杀虫活性不高的根本原因.
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| 关 键 词： | 苏云金芽孢杆菌 晶体含量 棉铃虫生物测定 |
Relationship between the crystal and bioactivity in different Bt strains isolated from different habitats |
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| Authors: | Pan Xiaohong Wang Feipeng Yao Rongying Wu Xiaofeng Sun Linying
Lin Zhiyi Guan Xiong |
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| Abstract: | Six new Bt strains, W14, ZY1, 3346, TMQ2, B31 and P91, were isolated from different habitats by NaAc-antibiotics method. Small- rhomboidal crystals were observed under microscope. SDS-PAGE showed that the molecular weights of the six new Bt strains were all about 130kDa. However, the contents of ICPs were only 0.2% to 0.29%. In addition, none of ICPs was found in B31. Bioassay results showed that the strains had low activities to Cotton Bollworm. We suggested that different sources and habitats infect the Bt ICPs contents and activities to target insects. |
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| Keywords: | Bacillus thuringiensiszz ICPszz Cotton Bollworm bioassayzz |
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