Zeylenone对急性淋巴细胞白血病细胞增殖及凋亡的影响

目的探讨Zeylenone体外抗急性淋巴细胞白血病(Acute Lymphoblastic Leukemia,ALL)效应及作用的可能机制。方法应用MTT法比较Zeylenone对肿瘤细胞、正常细胞增殖的影响;AO/EB染色观察其对ALL细胞(Reh、RS4;11)凋亡形态学的改变;流式细胞仪检测药物对细胞凋亡及细胞周期的影响。结果 Zeylenone对多种细胞呈现增殖抑制作用,且对ALL细胞株呈现更高的敏感性,而对外周血单个核细胞(Peripheral blood mononuclear cell,PBMC)抑制作用较小,抑制Reh、RS4;11细胞增殖呈时间依赖性和剂量依赖性;Zeylenone能够诱导ALL细胞凋亡,阻滞细胞周期于G0/G1期。结论 Zeylenone体外具有抗ALL细胞增殖的作用,其作用与诱导细胞凋亡、阻滞细胞周期相关。

Effects of Zeylenone on the proliferation and apoptosis of acute lymphoblastic leukemia cells

Abstract] Objective To explore the anti-ALL effects of Zeylenone and its potential mechanism. Methods The effects of Zeylenone on the proliferation of cancer cells and normal cells were tested by MTT assay. The morphological changes were boserved by AO/EB staining. Apoptosis and cell cycle of cells were determined by flow cytometry. Results Zeylenone inhibited the proliferation of many cell lines but had little toxicity to peripheral blood mononuclear cells(PBMC). All cell lines(Reh,RS4;11) showed relative sensitive to Zeylenone. The suppressive effects of Zeylenone on Reh and RS4;11 were in a dose-dependent and time-dependent manners. Zeylenone induced cell apoptosis and blocked cell cycle progression at G0/G1 phase. Conclusion The inhibition effects of Zeylenone on ALL cell proliferation may be played by inducing apoptosis and inhibiting cell cycle progression.