| 根癌农杆菌介导水稻遗传转化研究进展及展望 |
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| 作者姓名： | 宁约瑟 刘雄伦 戴良英 王国梁 |
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| 作者单位： | 湖南农业大学水稻基因组学实验室，湖南农业大学水稻基因组学实验室，湖南农业大学水稻基因组学实验室，湖南农业大学生物安全科技学院 长沙410128，湖南农业大学水稻基因组学实验室，长沙410128，长沙410128，长沙410128，长沙410128美国俄亥俄州立大学植物病理实验室43210 |
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| 基金项目： | 国家863项目“水稻抗稻瘟病基因Pi9的抗性机理研究和利用”(2005AA241010)，湖南农业大学人才基金项目(03WD04) |
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| 摘 要： | 自1994年建立了农杆菌介导的粳稻高效遗传转化体系以来,在短短十几年里取得了飞速发展。概述了农杆菌介导水稻遗传转化的研究历史和原理、影响农杆菌介导水稻转化体系的重要因素、转基因水稻的特点和外源基因的稳定性,并探讨了农杆菌介导水稻遗传转化研究中存在的问题与对策。
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| 关 键 词： | 根癌农杆菌 水稻 遗传转化 |
Advances and Perspectives of Agrobacterium tumefaciens-mediated Transformation in Rice |
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| Authors: | Ning Yuese Liu Xionglun Dai Liangying Wang Guoliang |
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| Institution: | 1,Rice Genomics Laboratory of Hunan Agricultural University, Changsha 410128;2,College of Bio-safety Science and Technology of Hunan Agricultural University, Changsha 410128; 3,Department of Plant Pathology, Ohio State University, Columbus, Ohio 43210 |
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| Abstract: | Since the establishment of the Agrobacterium tumefaciens-mediated rice transformation in 1994, many improvements have been made in the past ten years. In this review, the history and principles of the transformation method, genetic factors affecting transformation efficiency and stability of the foreign genes were discussed. Finally the challenges and perspectives of the useful transformation method for rice functional genomics and biotechnology were presented. |
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| Keywords: | Agrobacterium tumefaciens Rice Transformation |
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