| 农业生物技术在水稻种子纯度鉴定中的应用 |
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| 作者姓名： | 李小林 邓安凤 徐雨然 吴殿星 李健强 王建华 |
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| 作者单位： | 云南省农业科学院粮食作物研究所，中国农业大学农学与生物技术学院 北京100094，云南省农业科学院粮食作物研究所，云南省农业科学院粮食作物研究所，浙江大学原子核农业科学研究所，中国农业大学农学与生物技术学院，中国农业大学农学与生物技术学院，昆明650205，昆明650205，昆明650205，杭州310029，北京100094，北京100094 |
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| 基金项目： | 云南省省院省校科技合作项目“杂交水稻种子纯度快速检测和高效排假技术研究及示范”（2005XX28）.云南省农业综合开发项目“云南省高效‘放心’系列优质稻推广示范”（YFKJHZ-200610）. |
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| 摘 要： | 【研究目的】为了选择直观、准确、可靠、经济可行和易于操作的水稻种子纯度鉴定技术;【方法】分析和总结了电泳鉴定法、DNA分子标记鉴定法和叶色标记技术鉴定水稻种子纯度的研究进展及应用概况;【结论】作者认为电泳鉴定法及DNA分子标记鉴定法只能获得杂交水稻杂种纯度的基本数据,不具备有效排除假杂种的功能,也不可能采取后补救措施解决因纯度低而造成的产量损失。白化转绿型叶色标记技术运用在杂交水稻上,将结束传统种子纯度的检测方法,引发种子纯度检测的革命,具有极其广阔的市场前景。
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| 关 键 词： | 水稻种子 生物技术 叶色标记 纯度鉴定 |
Research Progress on Agricultural Biotechnology Utilizing in Purity Identification of Rice Seed |
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| Authors: | Li Xiaolin Deng Anfeng Xu Yuran Wu Dianxin Li Jianqiang Wang Jiahua |
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| Institution: | (1 Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650205; 2InstituteofNuclearAgriculturalSciences,ZhejiangUniversity, Hangzhou 310029;
3 Agronomy & Biotechnology College, China Agricultural University, Beijing 10094) |
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| Abstract: | Objectives]To select one technique to identify rice seed purity which was direct, accurate, reliable, economical and simple to manipulate.Methods]the research and utilizing status of rice seed purity identification by electrophoresis, DNA molecular markers and leaf-color marker were analyzed and summarized in the paper.Conclusion]It was considered that techniques of rice seed purity identification by electrophoresis and DNA molecular markers can only obtain the data of hybrid seed purity but distinguish the not real hybrid seed, consequently no measures to deal with the low yield due to low hybrid seed purity. The utilization of leaf-color marker of white to green will end the traditional techniques and lead to revolution of seed purity identification, which have a broad utilization in the practice. |
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| Keywords: | Rice seed Biotechnology Leaf-color marker Purity identification |
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