龙眼体胚发生中期发育同步化的初步调控

试验以龙眼"红核子"胚性愈伤组织为材料,对龙眼体细胞胚胎发生进行同步化调控,2,4-D浓度为0.07mg/L时获得了心形胚,2,4-D浓度为0.05mg/L和0.06mg/L时获得了鱼雷形胚,2,4-D浓度为0mg/L时获得了高度同步化的子叶形胚。研究表明,通过培养基中2,4-D浓度的微量调整,基本能够控制龙眼体胚发育中期心型、鱼雷形胚的发育同步化。

Preliminary synchronization regulation at the middle developmental stage during longan somatic embryogenesis

In this experiment the synchronization regulation of somatic embryogenesis from the embryogenic callus was performed in Dimocarpus longan Lour. cv. Honghezi. The somatic embryos at heart-stage were obtained when the concentration of 2,4-D was 0.07 mg/L, the somatic embryos at torpedo-stage were ob- tained when the concentrations of 2,4-D were 0.05 mg/L and 0.06 mg/L , the somatic embryos at cotyle- donary-stage were obtained when the concentration of 2,4-D was 0 mg/L. The results showed that the developmental synchronization of somatic embryos at heart-stage and torpedostage could be roughly achieved by changing the concentrations of 2,4-D on the media gently at the middle developmental stages during longan somatic embryogenesis.