电针对MPTP亚急性帕金森病模型小鼠步态运动行为的影响

目的 研究电针治疗早期对帕金森病（Parkinson''s disease,PD）小鼠模型步态运动行为的影响。方法 步态运动行为训练合格的C57BL/6小鼠40只,随机分为对照组、模型组、美多芭组和电针组,每组10只。对照组腹腔注射生理盐水,模型组、美多芭组和电针组腹腔注射MPTP诱导亚急性PD小鼠模型。对照组、模型组不予干预,美多芭组给予美多芭灌胃,电针组给予电针"合谷"太冲"干预,连续7 d后进行步态行为学实验、爬杆实验、悬挂实验、转棒实验。结果 模型组与对照组比较,平均体转角、单支撑时相、双支撑时相、足迹平均强度、对侧协调性、速度、平均步行周期、支撑时相、瞬时速度等步态指标均有明显变化（P<0.05,P<0.01）;与模型组比较,电针组、美多芭组的单支撑时相_左后、单支撑时相_右后、速度、平均步行周期、瞬时速度_左后、瞬时速度_右后、左前推进指数、右前推进指数等步态指标有明显改善（P<0.05,P<0.01）;模型组与对照组比较,下半部时间、总时间等爬杆指标均明显增加（P<0.01）;模型组与对照组比较,掉落潜伏期、在杆足数得分等悬挂指标均有明显下降（P<0.05,P<0.01）;模型组与对照组比较,转棒指标掉落潜伏期明显下降（P<0.05）;电针组、美多芭组与模型组比较,爬杆、悬挂、转棒指标均无明显变化（P>0.05）。结论 电针在治疗早期即可改善PD小鼠的步态运动行为,全自动小鼠步态分析系统能够在电针治疗早期准确敏感反映PD小鼠步态运动行为的细微改变。

Effects of Electroacupuncture on Gait Movement Behavior in MPTP Sub-acute Parkinson's Disease Model Mice

Objective To study the effects of electroacupuncture on gait movement behavior of Parkinson''s disease (PD) model mice at early stage. Methods A total of 40 C57BL/6 mice were randomly divided into a control group, a model group, a Madopar group and an electroacupuncture (EA) group, with 10 mice in each group. The control group was given intraperitoneal injection of normal saline. The sub-acute PD mice models were induced by the intraperitoneal injection of MPTP in the model group, the Madopar group and the EA group. The control group and model group were not intervened. The Madopar group was given intragastric administration of Madopar, and the EA group was given electroacupuncture intervention at "Hegu (LI 4)" and "Taichong (LR 3)". After 7 consecutive days, gait behavior experiment, rod climbing experiment, suspension experiment and rod rotation experiment were carried out. Results Compared with the control group, the gait indexes such as average body rotation angle, single support phase, double support phase, average footprint strength, contralateral coordination, speed, average walking cycle, support phase and instantaneous speed were significantly changed in the model group (P<0.05, P<0.01). Compared with the model group, the gait indexes of the EA group and the Madopar group were significantly improved (P<0.05, P<0.01), such as single support phase (left back), single support phase (right back), speed, average walking cycle, instantaneous speed (left back), instantaneous speed (right back), left forward propulsion index, right forward propulsion index, etc. (P<0.05, P<0.01). Compared with the control group, climbing rod indexes like the lower half time and the total time in the model group were increased significantly (P<0.01). Compared with the control group, the suspension indexes such as the drop latency and the number of feet on the rods in the model group were decreased significantly (P<0.05, P<0.01). While the drop latency of the rod rotation index was decreased significantly (P<0.05) in the model group compared with the control group. Compared with the model group, there were no significant changes in the indexes of climbing rod, suspension and rotating rod in the EA group and Madopar group (P>0.05). Conclusion Electroacupuncture can improve the gait movement behavior of PD mice in the early stage of treatment, and the automatic mice gait analysis system can accurately and sensitively reflect the subtle changes of the gait movement behavior of PD mice in the early stage of electroacupuncture treatment.