养阴润目丸对干眼模型大鼠结膜上皮细胞中Caspase-3、Caspase-6表达的影响

目的 研究养阴润目丸对干眼模型大鼠结膜上皮细胞中半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）、半胱氨酸天冬氨酸蛋白酶-6（Caspase-6）表达的影响。方法 将50只SD雄性大鼠按随机数字法分成5组，10只/组，分别为正常组、假手术组、模型组、养阴润目丸组、杞菊地黄丸组。模型组、养阴润目丸组、杞菊地黄丸组3组均采用去势法建立干眼模型。各组予以相应干预，干预30 d后，取大鼠结膜上皮组织，采用Tunel法检测Caspase-3、Caspase-6的阳性表达率。结果 养阴润目丸组和杞菊地黄丸组结膜上皮细胞中Caspase-3、Caspase-6的阳性表达明显低于模型组（P<0.05）。结论 养阴润目丸对干眼的作用机制可能是与降低结膜上皮细胞中Caspase-3、Caspase-6的表达活性从而阻断相关细胞凋亡级联反应相关。

Effects of Yangyin Runmu Pills on the Expression of Caspase-3 and Caspase-6 in Conjunctival Epithelial Cells in a Rat Model of Dry Eye

Objective To study the effects of Yangyin Runmu Pills on the expression of Cysteine aspartase-3 (Caspase-3) and Cysteine aspartase-6 (Caspase-6) in conjunctival epithelial cells in a rat model of dry eye. Methods A total of 50 Sprague-Dawley (SD) male rats were randomly divided into 5 groups:normal group, sham operation group, model group, Yangyin Runmu Pills group, Qiju Dihuang Pills group, with 10 rats in each group. The model group, the Yangyin Runmu Pills group and the Qiju Dihuang Pills group were prepared as the models of dry eye by using the method of ovariectomy. Each group was given corresponding intervention. After 30d intervention, the conjunctival epithelial tissues were taken to test the positive expressive rate of Caspase-3 and Caspase-6 by Tunel assay. Results The positive expressive rates of Caspase-3 and Caspase-6 in conjunctival epithelial cells in the Yangyin Runmu Pills group and the Qiju Dihuang Pills group were significantly lower than that of the model group (P<0.05). Conclusion The mechanism of action of Yangyin Runmu Pills on dry eye may be related to the inhibition of Caspase-3 and Caspase-6 expressive activities in conjunctival epithelial cells, thereby blocking the correlation of related apoptosis cascade reactions.