补肾、活血、祛痰法对激素性股骨头坏死模型血液流变学的影响

目的 观察补肾、活血、祛痰三种中医治法对激素性股骨头坏死模型兔血液流变学的影响。方法 在运用改良Matsui方法成功制备兔激素性股骨头坏死模型后，108只白兔随机分为正常对照组、模型对照组、通络生骨组、补肾组、活血组、祛痰组共6组，每组18只，通络生骨组、补肾组、活血组、祛痰组分别给予通络生骨胶囊、右归饮、桃红四物汤和苓桂术甘汤灌胃，其余各组则灌服同容量生理盐水。在实验第2周，第4周，第8周分别抽血检查血液流变学指标。结果 与正常对照组比较，模型对照组第2、4、8周血液流变学指标均维持在较高水平（P<0.01）。在第2周时，通络生骨组、活血组、祛痰组与模型对照组比较，均得到下降（P<0.05），其中又以通络生骨组、活血组改善更明显，该两组在实验第8周时恢复至正常白兔水平（P>0.05）。补肾组在实验第2周时较模型对照组改善不明显（P>0.05），至第4周时才有明显改善（P<0.05）。结论 桃红四物汤较右归饮、苓桂术甘汤能迅速改善激素性股骨头坏死血液流变学指标，右归饮、苓桂术甘汤对血液流变学指标的改善作用可能与其它途径相关，提示补肾、活血、祛痰三种中医治法治疗激素性股骨头坏死的作用机制可能不同。

Hemorheology of Steroid-Induced Femoral Head Necrosis with Methods:Tonifying Kidney,Activating Blood and Eliminating Phlegm

Objective To observe the three therapeutic methods, tonifying kidney, activating blood and eliminating phlegm on hemorheology of steroid-induced femoral head necrosis. Methods The steroid-induced femoral head necrosis rabbit models were successfully established by improved Matsui method. The 108 rabbits were randomly divided into normal control group, model control group, Tongluo Shenggu group, tonifying kidney group, activating blood group, eliminating phlegm group, 18 rabbits in each group. Rabbits of Tongluoshenggu group, tonifying kidney group, activating blood group, eliminating phlegm group were given by gavage with Tongluo Shenggu capsule, Yougui Yin, Taohong Siwu decoction, Linggui Zhugan decoction, respectively. The rest groups were given by gavage with the same dose normal saline. The hemorheology index after 2, 4, 8 weeks was detected. Results Compared with normal control group, the hemorheology indicators of model control group after 2, 4, 8 weeks increased (P<0.01). The hemorheology indicators of Tongluo Shenggu group, activating blood group and eliminating phlegm group were lower than model control group after 2 weeks (P<0.05), and the improvement in Tongluo Shenggu group and activating blood group was more obvious, which had reached normal levels at 8 weeks (P>0.05). There was no improvement in Tonifying kidney group after 2 weeks (P>0.05), However, it gained obvious improvement after 4 weeks (P<0.05). Conclusion Taohong Siwu decoction could more rapidly improve hemorheology indicators than Yougui Yin and Linggui Zhugan decoction on steroid-induced femoral head necrosis. The improvement of Yougui Yin and Lingguizhugan decoction may be related to other pathway. The mechanism of the three diffident therapeutic methods, tonifying kidney, activating blood and eliminating phlegm on steroid-induced femoral head necrosis may be different.