NaCl、KNO3处理对辣椒种子发芽及其幼苗同工酶的影响

以不同浓度的NaCl、KNO3溶液处理4个辣椒品种种子,探讨了各溶液对辣椒种子的发芽率、发芽势及发芽指数的影响,研究了不同处理条件下萌发芽苗体内POD、SOD同工酶的变化。结果表明:不同品种的发芽率、发芽势和发芽指数差异显著,天鹰和改良线椒8819的上述3项指标都处于较高的水平,天鹰在受到NaCl处理时的发芽率和受到KNO3处理时的发芽率表现截然相反的趋势,春雨在0.3%KNO3处理下有了71.4%的最大发芽率,用蒸馏水处理改良线椒8819更有利于发芽;相对CK而言,4个品种辣椒种子在NaCl、KNO3处理下,其POD同工酶谱带的带数增加,染色加深,而它们在受到同浓度离子的不同处理时,SOD同工酶酶带的亮度高低为:NaCl>KNO3>CK,说明高浓度的NaCl处理能显著提高辣椒种子的SOD同工酶活力。

Influence of NaCl and KNO3 treatment on pepper seed germination and isoenzyme activity

Influence of different concentration of NaCl and KNO3 treatment on 4 kinds of pepper seed germination and its isoenzyme activity was studied.The results showed that pepper of different kind made obvious distinction in germination percentage,viability,index and activity of POD,SOD,in which Tianying and reforming string-pepper 8819 were in higher level.Tianying showed contrary tendency of germination percentage when treating by NaCl and KNO3.Chunyu got 71.4%,the biggest germination percentage by 0.3% KNO3 treatment,and distilled water could accelerate reforming string-pepper 8819 germination.Comparing to CK,POD isoenzyme bands of 4 kinds of pepper improved and dyed deeper,and under NaCl treatment,SOD isoenzyme bands was brighter than by KNO3,which indicated that NaCl could enhance SOD isoenzyme activity remarkably.