| 转枯草芽孢杆菌纤溶酶基因对烟草氧自由基和保护酶系统的影响 |
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| 作者姓名： | 李国婧 姜树原 吴自荣 王水平 王瑞刚 |
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| 作者单位： | (1内蒙古农业大学生物工程学院，呼和浩特 010018；2内蒙古农业大学甜菜生理研究所，呼和浩特 010018；3华东师范大学生命科学学院，上海 200062；4内蒙古农业大学农学院 呼和浩特 010018) |
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| 基金项目： | 上海市科委98重大项目"安舒克栓酶的药效及工艺研究"(编号:985419000-3). |
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| 摘 要： | 除个别时期外，转枯草芽孢杆菌纤溶酶(Bacillus subtilis fibrinolytic enzyme,BSFE)基因及其信号肽和前肽序列烟草叶、茎、根氧自由基含量、超氧化物歧化酶活性均显著高于野生型烟草植株，抗坏血酸过氧化物酶活性也较野生型烟草植株高，但二者差异不显著。推测氧自由基含量的变化与BSFE的蛋白水解活性有关；超氧化物歧化酶和抗坏血酸过氧化物酶活性变化可能与该转基因烟草植株对外源BSFE基因表达和产物累积产生的逆境适应有关。
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| 关 键 词： | 枯草芽孢杆菌纤溶酶(Bacillus subtilis fibrinolytic enzyme BSFE) 转基因烟草 SOD Vc过氧化物酶 氧自由基 |
Influence of Trans-Bacillus subtilis fibrinolytic enzymic gene on Oxygen Radical and Protective Enzyme System in Tobacco |
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| Authors: | Li Guojing Jiang Shuyuan Wu Zirong Wang Shuiping Wang Ruigang |
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| Institution: | (1 College of biology engineering ,Inner Mongolia Agricultural University, Hohhot 010018
2 Sugarbeet Physiological Institute, Inner Mongolia Agricultural University, Hohhot 010018
3 School of Life Science,East China Normal University,Shanghai 200062
4 The |
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| Abstract: | Except one or two period, the quantitiy of oxygen radical and the activities of superoxide dismatase and ascorbic acid peroxidase in the leaves, stems and roots of trans-BSFE gene and its signal peptide and propeptide sequence tobacco were all higher than those of the common tobacco. We infer the change of the quantity of oxygen radical relates with the hydrolyzing activity of BSFE protein, and the change of activities of superoxide dismutase and ascorbic acid hydrogen peroxidase ralate with counter-adaptation to the expression of exogenons BSFE gene and its product accumulation of the transgenic tobacco. |
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| Keywords: | BSFE Transgenic tobacco SOD Vc peroxidase Oxygen radical |
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