不同光质对菊花组培苗生长的影响

【研究目的】该文研究新型光源LED辐射的不同光质对菊花组培苗生长的影响,以期为植物组培专用LED光源的研发提供数据支持和理论依据。【方法】以菊花组培苗为试材,采用LEDs光源发射的单色光谱红光(625±20)nm]、蓝光(460±20)nm]、远红光(730±20)nm]和绿光(530±20)nm],进行不同光质配比组合,荧光灯作为对照,对组培苗形态、生根,色素含量,碳氮代谢及抗氧化酶系活性进行差异比较。【结果】菊花组培苗在红光下徒长,能效最大。蓝光下矮壮,根系活力最大,复合LEDs光质下,组培苗形态正常。RBG处理的菊花组培苗叶片色素含量最高。红光有利于叶绿素b的合成,蓝光有利于叶绿素a的合成。单频红光处理的菊花叶片淀粉含量最高,RBG处理的叶片可溶性糖、碳水化合物蔗糖、游离氨基酸含量最高。蓝光有利于蛋白质的合成,LEDs光质处理的叶片C/N比高于荧光灯。【结论】LEDs光源系统将成为植物组织培养的理想光源。

Different Light Qualities on Growth of Chrysanthemum (Dendranthema morifolium)Plantlets in virto

OBJECTIVE]The effects of different light qualities on growth of chrysanthemum in vitro under light-emitting diodes(LEDs)were investigated.METHOD]By arraying the different wave length and ratio of LEDs,the 7 kinds of light quality were built.The morphological and physiological characteristic,such as rooting,pigment content,carbon-nitrogen metabolism and antioxidative enzymes activities of chrysanthemum plantlets breeding 32-12 under the 7 kinds of light quality,were compared with fluorescent lamp.RESULTS]The results showed that greatest stem length and the highest energy efficiency was obtained under monochromatic red light.Under blue light,the plantlets were low and strong,and root vigor was highest.Plants grown in complex of LEDs showed normal growth.Pigments under RBG were higher than other treatments.Red light promoted the contents of Chl b,blue light promoted the contents of Chl a.The contents of starch in leaf is highest under monochromatic red light,RBG light stimulated the accumulation of soluble sugar,carbon hydrate,Amino acid Soluble.Blue light benefits to synthesize protein.Under LEDs,C/N ratios is higher than fluorescent lamp.CONCLUSION]It is reasonable to expect that the total LEDs irradiation system will be the major light source for plant tissue culture in future.