心血瘀阻证动态演变过程生物信息学研究

目的 探讨冠心病心血瘀阻证动态演变过程中血瘀证前期、亚血瘀证期、心血瘀阻证期3个阶段的代谢组学通路。方法 信号通路分析采用KEGG，代谢产物分子注释、相关的酶或转运蛋白及其相关性质分析采用HMDB，代谢产物路径可视化采用metPA网络软件。结果 用MetPA分析的生物代谢通路显示，12个代谢产物参与了22条代谢路径。其中6条通路（氨酰-tRNA生物合成、缬氨酸，亮氨酸和异亮氨酸生物合成、缬氨酸，亮氨酸和异亮氨酸生物合成及降解、精氨酸和脯氨酸代谢、半乳糖代谢，D-精氨酸与D-鸟氨酸代谢）的影响值P<0.05。结论 6条密切相关的通路主要集中在亚血瘀证期和心血瘀阻期。

Research on Dynamic Evolution of Heart Blood Stasis Syndrome Based on Bioinformatics

Objective To investigate the three stages of metabolic pathways on early-stage of blood stasis syndrome, sub-stage of blood stasis syndrome and stage of heart blood stasis syndrome during the dynamic evolution of heart blood stasis syndrome in patients with coronary heart disease.Methods Signal pathways were analyzed by using KEGG. Molecular annotations of metabolites, related enzymes or transporters and their associated properties were analyzed by using HMDB. Metabolic pathways visualization were analyzed by using metPA network software.Results Metabolic pathways showed that 12 metabolites were involved in 22 metabolic pathways. The influence value of 6 pathways (biosynthesis of aminoacyl-tRNA, biosynthesis of valine, leucine and isoleucine, degradation of valine, leucine and isoleucine, metabolism of arginine and proline, metabolism of galactose, metabolism of D-Arginine and D-ornithine) was P<0.05.Conclusion The six closely related pathways are mainly concentrated in sub-stage of blood stasis syndrome and stage of heart blood stasis syndrome.