羊乳腺上皮细胞的形态观察

用组织块培养法获得山羊乳腺细胞原代培养物,并根据山羊乳腺成纤维细胞与上皮细胞对胰蛋白酶的敏感性不同将二者分离纯化。对细胞形态进行光镜观察发现:纯化的山羊乳腺上皮细胞传代至第15代时其生长仍正常。山羊乳腺上皮细胞含不同的细胞类型,大多数上皮细胞呈短梭形或多角形,呈蜂窝状;部分细胞呈圆饼状,体积较大;部分细胞呈长形。纯化的乳腺上皮细胞增殖可形成圆顶型结构,呈乳头状,称之为乳球体。

Observed on the characteristics of goat mammary epithelial cells

Primary culture of goat mammary gland cells could be achieved by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be purified according to their different sensibility to trypsin. The characteristics of Goat mammary epithelial cells were studied by light microscope. The results were as follows: The purified goat mammary epithelial cells kept normal characteristics until 15th passages. The goat mammary epithelial cells contained different cell type, majority of the cells were short shuttle-like or guboidal, they looked like beehive; part of the cells looked like big round flat cakes; part of the cells were long. The purified Mammary epithelial cells propagated and formed dome-like construction which looked like nipple and it was called milk orb.