| 白鹤芋花丝体细胞胚的诱导及快繁技术 |
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| 作者姓名： | 秦静远 |
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| 作者单位： | 杨凌职业技术学院 陕西杨凌712100 |
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| 基金项目： | 杨凌职业技术学院--比利时佛拉芒国外教育计划协会（YLVTC-VV0B）园艺合作项目基金资助. |
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| 摘 要： | 适合白鹤芋两种基因型的雄蕊花丝体细胞胚诱导的培养基是MS TDZ0.05mg/L NAA1.5mg/L,体细胞胚发生于花丝基部。幼嫩的雄蕊,其花丝细胞较敏感,易于诱导体细胞胚的发生。42d后,将形成的体细胞胚转接至培养基MS 6-BA2mg/L NAA0.05mg/L上诱导次生体细胞胚发生。再过42d,它们就可以诱导成苗。最好的诱导体细胞胚成苗培养基是MS无激素培养基。
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| 关 键 词： | 白鹤芋 花丝 胚胎发生 快繁 |
Induction of Somatic Embryogenesis on the Anther Filament and Rapid Propagation of Spathiphyllum cannifolium. L. |
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| Authors: | Qin Jingyuan |
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| Institution: | Yartgling Vocational and Technical College, Yangling Shaanxi 712100 |
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| Abstract: | When placed on a MS medium supplemented with 0.05mg/LTDZ and 1.5mg/LNAA, anthers of two spathiphyllum genotypes develop clumps of somatic embryos on the filament. The younger anthers, the more sensitive their filament cells were for the induction of somatic embryogenesis. After 42days, the somatic embryo were transferred to a MS medium supplemented with 2mg/L6-BA and 0.05mg/LNAA, A develop secondary somatic embryos. Another 42days later, they were ready for conversion. The best conversion medium was a hormone-free MS medium. |
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| Keywords: | Spathiphyllum Filament Embeyogensis Rapid Propagation |
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