海藻糖在渗透胁迫下对油菜种子萌发和幼苗生理的影响

研究了在25%PEG-6000渗透胁迫下海藻糖(TH)对油菜种子萌发和幼苗生理的影响。结果表明:25mmol/LTH能明显促进渗透胁迫下油菜种子的萌发和幼苗生长,种子发芽率提高19.52%、发芽指数提高33.12%、活力指数提高1.76倍;幼苗MDA含量下降37.77%,Pro含量提高6.96%;POD活性提高33.11%,SOD活性提高1.4倍,CAT活性提高51.08%。

Influence of Trehalose on Rapeseed Germination and Seedlings Physiology under Osmotic Stress

Seeds and seedlings of rape variety Yuhuang No.1 were treated with 25 %PEG-6000 in combination with different concentrations (5 and 25 m mol/L) of trehalose (TH) and control to study the influence of external TH on rapeseed germination and seedlings growth and physiology under the osmotic stress. The results showed that 25 m mol/L TH could promote germination of rapeseed and seedlings growth under the osmotic stress, and the germination rate increased by 19.52%, the germination index increased by 33.12% and the vigor index increased by 1.76 times. At the same time, 25 m mol/L TH alleviated the accumulation of MDA by 37.77 % and accelerated the accumulation of proline by 6.96 %, and significantly enhanced the activity of peroxidase (POD) by 33.11%, superoxide dismutase (SOD) by 1.4 times and catalase (CAT) by 51.08 %. In comparison with these changes, the effects of lower concentration of TH (5 m mol/L) and corresponding control were not so obvious. The above results indicate that higher concentration of TH (25 m mol/L) could promote the germination of rapeseed and the seedlings growth under the osmotic stress.