烟草感染青枯菌前后POD及同工酶的变化

【研究目的】对烟草感染青枯菌前后的根、茎、叶内过氧化物酶(POD)及同工酶进行比较研究,为抗病育种提供理论基础。【方法】采用比色法测POD酶活,用聚丙烯酰胺凝胶电泳(PAGE)进行POD同工酶的比较。【结果】对烟草感染青枯菌前后,酶活性测定结果表明,烟草叶部POD活性显著高于根部和茎部。烟草接菌后,根、茎、叶内的POD活性均在第3天升至最高,而叶内比对照增高了29.67%。同工酶电泳分析表明,烟草接菌后的同工酶谱带,在条带的数目、种类和各条的相对活性上表现出差异。【结论】POD作为植物的保护酶类,随植物抵抗疾病的过程变化而变化,故与植物抗病性密切相关。

Changes of Peroxidase and Isoenzyme in Tobacco Infected by Pseudomonas Solanacearum

OBJECTIVE]Comparatively peroxidase (POD) and isoenzyme of roots?stems and leaves in tobacco infected with Pseudomonas solanacearum were studied in order to offer academic foundations for breeding resistive plants. METHOD] Colorimetry for POD activity and polypropylene amic gel electrophoresis(PAGE)for isoenzyme were used.RESULTS]After inoculated by Pseudomonas Solanacearum, It was found there was the highest peroxidase(POD) activity in leaves than in roots and in stems, and the POD activity in roots?stems and leaves was highest in 3 days but in leaves which improved 29.67% percent than those form the contrast. Isoenzyme electrophoresis showed that difference occurred in numbers and types of isoenzyme bands and related activity of every band between different samples. CONCLUSION]POD was a protectal enzyme and it was changing with infection , so it was suggested that POD may be close relation to resistance against disease.