转ScMV-CP基因甘蔗对根际土壤酶活性及微生物的影响

【:研究目的】转ScMV-CP基因甘蔗对根际土壤酶活性和微生物的影响。【方法】利用BIOLOG EcoplateTM微平板(BIOLOG Inc.,USA)结合微生物的平板培养及相关的土壤酶活性分析【。结果】结果表明:转基因甘蔗显著(p<0.05)降低了土壤脱氢酶的活性,提高了土壤的纤维素酶、蛋白酶和脲酶活性(p<0.01),而对蔗糖酶和磷酸单脂酶没有影响;转基因甘蔗显著增加了根际土壤可培养的细菌和真菌数量(CFUs),但对放线菌影响很小。转基因甘蔗并没有增加土壤中对卡那抗性的微生物数量(CFUs);BIOLOGEcoplate研究表明转基因甘蔗稍微提高了根际土壤细菌的代谢活力,细菌多样性分析表明,根际土壤细菌的Simpson指数(D’)、Shannon-Wiener指数(H’)、细菌生理群分布的均匀度、Mclntosh指数(DMc)差异不明显【。结论】转基因甘蔗对根际土壤酶和土壤微生物有一定影响,但没有增加对卡那抗性的微生物数量(CFUs);BIOLOG实验未发现转基因甘蔗对土壤细菌的多样性有影响。

Effects on Enzyme Activities and Microbe in Rhizosphere soil of ScMV-CP transgenic Sugarcane

OBJECTIVE]Study on effects on enzyme activities and microbe in rhizosphere soil of ScMV-CP transgenic sugarcane. METHOD]This experiment used BIOLOG Ecoplate and plate cultivation of microorganism associated with related soil enzyme activities analyses under field release of transgenic sugarcane. RESULTS]The results showed that the dehydrogenase activity was lowered significantly(p<0.05), and the cellulose, protease and urease activities were improved remarkably(p<0.01), but there was no difference on sucrase activity and phosphatase activities in rhizosphere soil of ScMV-CP transgenic sugarcane compared with non-transgenic sugarcane. The total number of colony forming units (CFUs) of culturable bacteria and fungi were increased significantly ((p<0.01) in transgenic sugarcane rhizosphere soil, but the total number of CFUs of culturable was not effected. No significantly higher CFUs of bacteria, fungi and actinomycetes resistant to kanamycin(Km)were obtained in transgenic sugarcane soil. BIOLOG experiment showed the metabolism activity of soil bacteria was increased lightly, and the soil bacteria diversity indexes such as Simpson index (D''), Shannon-Wiener index (H''), evenness, Mclntosh index (DMc) were not different from each other after inoculating 72h in BIOLOG Ecoplate.CONCLUSION]There were some effects on soil enzyme activities and CFUs of soil microbe in the rhizosphere soil of ScMV-CP transgenic sugarcane, but no increasement of CFUs of soil microbe resistant to kanamycin(Km)were obtained. And results BIOLOG experiment showed the transgenic sugarcane didn''t change the soil bacteria diversity.