蒲公英二倍体与四倍体的几个生理指标比较

【研究目的】通过测定和比较二倍体和四倍体蒲公英不同部位(根、叶、花序)的几个生理指标,包括类黄酮、异黄酮、Vc、可溶性糖含量、SOD、POD和CAT,探索药用蒲公英倍性育种的应用前景,为药用植物的多倍体诱导提供理论支持。【方法】以人工选育的四倍体药蒲公英为材料,以二倍体多裂蒲公英为对照,采用相关的方法进行分别测定。【结果】四倍体药蒲公英的这些指标比二倍体蒲公英都有所增加,其中类黄酮平均增加46%,异黄酮平均增加38%,Vc平均增加54%,可溶性糖平均增加50%,SOD平均增加29%,POD平均增加45%,CAT平均增加40%。【结论】人工四倍体药蒲公英的这些生理指标显示出较强的抗性基础,可望直接利用或作为进一步培育药蒲公英新品种的良好育种材料。

Comparison of Several Physiological Indexes in Diploid and Tetraploid Plant of T. mongolicum Hand

Objective]By comparing the several physiological indexes(flavonoid, isoflavone, Vc, the soluble sugar, SOD, POD , CAT) between the artificial tetraploid and the wild diploid plant of T. mongolicum Hand at different spots(root, leaf, anthotaxy), it is aimed at revealing the potential utility of the polyploidy in medicinal T.mongolicum Hand and providing the theory support for medicinal plant''s polyploid mutation. Methods]The artificial tetraploid plant of T. Officinale F. H. Wigg and the wild diploid plant of T. licentii V. Soest was used as materials in this study. The several physiological indexes was determined by adopting related method. Result] The result is that tetraploid plant shows higher level of flavonoid, isoflavone, Vc, the soluble sugar, SOD, POD and CAT than those of the diploid plant. These indexes of flavonoid, isoflavone,,Vc, the soluble sugar, SOD, POD, CAT in tetraploid plant respectively increased to 46%,38%,54%,50%,29%,45%,40% as Compared with diploid plant in average.Conclusion]The artificial tetraploid plant of T. Officinale F. H. Wigg presents great potential in stress resistance, which would be available in good seed breeding for new varieties of T.mongolicum Hand.