知柏地黄汤对UU感染大鼠生精细胞凋亡及凋亡因子Cyt-c、AIF表达的影响

目的 探讨知柏地黄汤对解脲支原体（Ureaplasma urealyticum，UU）感染大鼠精液质量、生精细胞凋亡及凋亡因子Cyt-c、AIF表达的影响。方法 从60只雄性SD大鼠中随机抽取45只，经膀胱注射造成UU感染动物模型，剩余15只作为正常组。UU感染模型动物再随机分成模型组、阿奇霉素组（西药组）和知柏地黄汤组（中药组），于接种后第10 d予以模型组和正常组生理盐水灌胃，阿奇霉素组和知柏地黄汤治疗组分别予相应药物灌胃，连续干预21 d后处死动物，检测各组精子运动参数、生精细胞凋亡率及细胞凋亡因子细胞色素C（cytochrome c，Cyt-c）、细胞凋亡诱导因子（apoptosis inducing factor，AIF）表达水平。结果 与正常组比较，模型组大鼠精子质量明显降低，UU培养阳性率、生精细胞凋亡率及Cyt-c、AIF表达明显升高（P<0.01，P<0.05）。与模型组比较，中药组、西药组精子质量提高，UU培养阳性率、细胞凋亡率、Cyt-c、AIF表达均降低（P<0.01，P<0.05）；与西药组比较，中药组改善精子质量较高（P<0.05，P<0.01），降低UU感染率，差异无统计学意义（P>0.05）。结论 知柏地黄汤改善UU感染大鼠精子质量优于阿奇霉素，且与阿奇霉素抗UU感染作用相当，其机制可能与其降低凋亡因子Cyt-c、AIF表达相关。

Effects of Zhibai Dihuang Decoction on Spermatogenic Cell Apoptosis of UU Infected Rats and Apoptotic Factors Cyt-c and AIF Expression

Objective To investigate the effect of Zhibai Dihuang Decoction on semen quality, spermatogenic cell apoptosis and apoptosis factors Cyt-c, AIF expression of Ureaplasma urealyticum (UU) infected rats. Methods 45 rats were randomly selected from 60 male SD rats, which were injected with UU by bladder to establish the infective animal models, and the remaining 15 rats were used as normal group. UU infection animal models were randomly divided into model group, azithromycin group (Western medicine group) and Zhibai Dihuang Decoction group (Chinese medicine group), on 10 d after inoculation, the model group and normal group were administrated with saline by gavage, azithromycin group and Zhibai Dihuang Decoction in the treatment group were given corresponding medicine gavage, the animals were executed after continuous intragastric administration for 21 days. The sperm motion parameters, germ cell apoptosis rate and cell apoptosis factor Cytochrome c(Cyt-c) and apoptosis inducing factor(AIF) expression level were detected. Results Compared with the normal group, the sperm quality of the model group was significantly decreased, the positive rate of UU, the apoptosis rate of the cells and the expression of Cyt-c and AIF were significantly increased (P<0.01, P<0.05). Compared with the model group, the sperm quality of Chinese medicine group and Western medicine group were improved, the positive rate of UU, the apoptosis rate, AIF and Cyt-c expression were decreased obviously (P<0.01, P<0.05). Compared with Western medicine group, the sperm quality in Chinese medicine group improved obviously (P<0.05, P<0.01), and lower UU infection rate was not statistically significant (P>0.05). Conclusion Zhibai Dihuang Decoction in improving sperm quality of UU infected rats is better than azithromycin, and its effect on resistant UU infection is equal to azithromycin.The mechanism may be related to decreased expressions of apoptosis factor cytochrome c and AIF.