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| 甘露寡糖对肉仔鸡肠道微生物区系发育的影响 | |
| 引用本文： | 温若竹,江芸,刘泽兴,程灵豪,邓宇,高峰,周光宏.甘露寡糖对肉仔鸡肠道微生物区系发育的影响[J].浙江大学学报(农业与生命科学版),2011,37(1). |
| 作者姓名： | 温若竹 江芸 刘泽兴 程灵豪 邓宇 高峰 周光宏 |
| 作者单位： | 1. 南京农业大学,动物科技学院,江苏,南京,210095 2. 南京师范大学,金陵女子学院,江苏,南京,210097;南京农业大学,教育部肉品加工与质量控制重点开放实验室,江苏,南京,210095 3. 南京农业大学,动物科技学院,江苏,南京,210095;南京农业大学,教育部肉品加工与质量控制重点开放实验室,江苏,南京,210095 4. 南京农业大学,教育部肉品加工与质量控制重点开放实验室,江苏,南京,210095 |
| 基金项目： | 江苏省青蓝工程资助项目 |
| 摘 要： | 利用PCR-DGGE技术对肠道细菌16S rDNA的V6-V8可变区进行分析,研究肉仔鸡生长过程中肠道微生物区系的发育规律,并重点探讨日粮中添加甘露寡糖对肉仔鸡肠道茵群稳定性及多样性的影响.选择1日龄健康AA肉仔鸡192羽,随机分为2组,Ⅰ组为对照组(饲喂基础日粮),Ⅱ组为甘露寡糖(MOS)组(1～21 d:饲喂基础日粮+0.2%MOS;22～42 d:饲喂基础日粮+0.1%MOS),每组4个重复,每个重复24羽.结果表明:肉鸡肠道茵群以乳酸杆菌为主要优势茵群,并且随着日龄变化各消化道的优势菌群发生相应改变;盲肠内菌群的多样性在各日龄段的表现最为丰富,且越接近消化道远端,各日龄间菌群的相似性就越低;日粮中添加MOS增加了肠道中乳酸杆菌等有益菌的数量,并可抑制梭菌属等有害菌的生长,维持肠道健康;同时还发现空白组各日龄肌胃、十二指肠、空肠、回肠和盲肠中菌群相似性最高分别可达77%、68%、72%、49%和63%,MOS组相应的分别为76%、84%、72%、49%和63%,这说明日粮中添加MOS会对肉鸡肠道茵群产生影响,但日龄的变化仍是影响肠道微生物区系的主要因素. |
| 关 键 词： | 肉鸡 肠道 甘露寡糖 微生物 变性梯度凝胶电泳(DGGE) |
Effects of mannose-oligosaccharide on bacterial community of gut in broilers |
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| WEN Ruo-zhu,JIANG Yun,LIU Ze-xing,CHENG Ling-hao,DENG Yu,GAO Feng,ZHOU Guang-hong.Effects of mannose-oligosaccharide on bacterial community of gut in broilers[J].Journal of Zhejiang University(Agriculture & Life Sciences),2011,37(1). | |
| Authors: | WEN Ruo-zhu JIANG Yun LIU Ze-xing CHENG Ling-hao DENG Yu GAO Feng ZHOU Guang-hong |
| Institution: | WEN Ruo-zhu1,JIANG Yun2,3,LIU Ze-xing1,CHENG Ling-hao1,DENG Yu1,GAO Feng1,ZHOU Guang-hong3(1.College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China,2.Ginling College,Nanjing Normal University,Nanjing 210097,3.Key Laboratory of Meat Processing and Quality Control,Ministry of Education,China) |
| Abstract: | DNA was extracted from the broiler's faecal samples and the V6-V8 regions of bacterial 16S rDNA were amplified and analyzed by denaturing gradient gel electrophoresis(DGGE) to monitor the development of bacterial community in faeces of broilers and the effects of mannose-oligosaccharide supplementation on bacterial community of intestinal tract.The broiler chickens were randomly assigned to 2 dietary treatments: Group I(control) was fed corn-soybean meal based diet,and group II was fed basal diet supplement... |
| Keywords: | broilers gut mannose-oligosaccharide(MOS) bacterial community denaturing gradient gel electrophoresis(DGGE) |
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