萼状粒毛盘菌多糖的生物活性

研究萼状粒毛盘菌YM-262胞内、胞外多糖对亚硝基的清除作用、对丙二醛生成的抑制作用、对线粒体的保护作用和抗炎作用。结果表明:胞内、胞外多糖质量浓度为1.2g·L-1时对亚硝酸盐的清除率可达最大,分别为63.21%和52.43%;胞内、胞外多糖均能显著抑制肝匀浆在自氧化和诱导氧化中丙二醛的生成,抑制率分别为32.81%和36.46%;此外,YM-262多糖可抑制.OH所致的氧化损伤,减轻线粒体膜肿胀度,且胞内多糖的抑制率大于胞外多糖;胞内多糖和胞外多糖均可显著抑制二甲苯所致的小鼠耳廓肿胀,减少左右耳质量差,且具有一定的剂量-效应关系,胞内多糖和胞外多糖剂量为400mg.kg-1时,抑制率分别为56.68%和51.87%。

Biological activity of polysaccharides produced by Lachnum calyculiforme

The effects of intracellular polysaccharide(LIP) and extracellular polysaccharide(LEP) from Lachnum calyculiforme YM-262 on the elimination of nitrite radicals,the inhibition of MDA formation,the protective action of mitochondria,and the anti-inflammation were studied.The results indicated that the clearance rates of nitrite could reach 63.21% and 52.43% at 1.2 g·L-1 of LIP and LEP,respectively.LIP and LEP could significantly inhibit the amount of malondialdehyde which was produced by liver homogenate in the self-induced oxidation and the induced oxidation.The inhibition rate of malondialdehyde were 32.81% and 36.46% at 1.2 g·L-1 of LIP and LEP,respectively.In addition,polysaccharides from L.calyculiforme YM-262 could inhibit the oxidative damage made by ·OH and reduce the swelling degree of mitochondrial membrane,and the LIP’s inhibitory rate was higher than the LEP’s.LIP and LEP could significantly inhibit the auricle tumefaction of mice and reduce the mass difference between the right and the left ear,which had certain dose-effect relations.The anti-inflammatory action could reach 56.68% and 51.87% with 400 mg·kg-1 of LIP and LEP,respectively.