枝状枝孢菌(Cladosporium cladosporioides)发酵产酶培养基的响应面法优化

采用响应面法对枝状枝孢菌(Cladosporium cladosporioides)产葡聚糖内切酶(CMCase)的液体发酵培养基进行优化。用单因素试验确定发酵培养基的最佳碳源、氮源和无机盐;通过中心组合试验和响应面法优化以上因素,得到的优化发酵培养基为:麦秸23.18 g.L-1、麦麸30.00 g.L-1、酵母膏13.62 g.L-1、KH2PO44.60 g.L-1、NaCl 4.60 g.L-1、MgSO4.7 H2O 0.46 g.L-1。在此条件下,该菌发酵液中CMCase活力达2.80 U.mL-1,较优化前提高了2.18倍。

Response surface optimization of medium components for producing cellulase by Cladosporium cladosporioides F4-1

The medium of liquid fermentation for producing CMCase (carboxymethyl cellulase) by Cladosporium cladosporioides strain F4-1 was optimized using response surface methodology (RSM). The optimum carbon, nitrogen and inorganic salts were obtained with one-factor-at-a-time. A central composite design and RSM were applied to determine the optimal concentration of each component above. The results show that the optimal medium was as follows:wheat straw 23.18 g·L～(-1),wheat bran 30.00 g·L～(-1), yeast extract 13.62 g·L～(-1),KH_2PO_4 4.60 g·L～(-1),NaCl 4.60 g·L～(-1),MgSO_4·7H_2O 0.46 g·L～(-1) . Under this condition, the CMCase activity reached 2.80 U·mL～(-1), which increased 2.18 times than before optimization.