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白菜核雄性不育两用系花蕾的mRNA差别显示及其cDNA差异片段分析
引用本文:曹家树,叶纨芝,张明,曾广文.白菜核雄性不育两用系花蕾的mRNA差别显示及其cDNA差异片段分析[J].浙江大学学报(农业与生命科学版),2001,27(6):596-600.
作者姓名:曹家树  叶纨芝  张明  曾广文
作者单位:浙江大学蔬菜研究所,
基金项目:国家自然科学基金资助项目(39670512)
摘    要:用mRNA差别显示技术对白菜核雄性不育两用系中不育株群与可育株群花蕾总RNA进行了比较分析.在6种反转录模板和20种差异随机引物共120种组合的选择扩增中共回收了18个cDNA差异片段,对其中6个cDNA差异片段进行Northern验证,获得了一个白菜核雄性不育两用系可育株群并在花蕾中特异表达的阳性差异片段,简称为B0302-4.Northern验证结果表明它在不育株中的表达受到了明显的抑制,其长度为463 bp.经GenBank BLAST查询,它与拟南芥第一条染色体上编码细胞色素P450的基因CYP86C4核苷酸序列有84.3%的同源性.

关 键 词:白菜  核雄性不育  mRNA差别显示
文章编号:1008-9209(2001)06-0596-05

Differential display of flower bud mRNA of genic male sterility (GMS) AB line in Chinese cabbagepak-choi and analysis of differential cDNA fragments
CAO Jia-shu,YE Wan-zhi,ZHANG Ming,ZENG Guang-wen.Differential display of flower bud mRNA of genic male sterility (GMS) AB line in Chinese cabbagepak-choi and analysis of differential cDNA fragments[J].Journal of Zhejiang University(Agriculture & Life Sciences),2001,27(6):596-600.
Authors:CAO Jia-shu  YE Wan-zhi  ZHANG Ming  ZENG Guang-wen
Abstract:The difference was compared by mRNA differential display technology in Chinese cabbage-pak-choi (Brassica campestris ssp. chinensis Makino var. communis Tsen et Lee) flower bud among genic male sterility (GMS) line (A line) and maintainer line (B line). 18 differential fragments have been obtained in total of 120 selective amplifications. A differential fragment named as B0302-4 was confirmed by Northern hybridization out of 6 cDNA differential fragments tested. B0302-4 was shared by the maintainer line, the expression of B0302-4 was restricted in the GMS line, and B0302-4 expressed specially in flower bud. Fragment B0302-4 is composed of 463 base pair. The result of GenBank BLAST showed that it has 84.3% nucleotide similarity to the CYP86C4 gene encoding cytochrome P450 from Arabidopsis.
Keywords:Chinese cabbage-pak-choi (Brassica  campestris ssp  chinensis Makino var  communis Tsen et Lee)  genic male sterility  mRNA differential display
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