WHBE兔遗传特异性的RAPD分析

应用随机扩增多态DNA技术(RAPD)对WHBE兔、日本大耳白兔和新西兰兔进行了遗传分析.用10个随机引物对其基因组DNA进行PCR扩增.根据电泳结果选用其中8个引物扩增的条带进行遗传分析,共检测到107条扩增片段,其中多态性片段32条,占29.91%,反映了家兔品系间的遗传变异.多态性统计分析表明,WHBE免和日本大耳白兔的遗传相似度最高,为0.7948.其中4个引物既可扩增出3个品系共同的DNA条带,也可扩增出不同品系的特征条带,表明WHBE兔与日本大耳白兔和新西兰兔之间有遗传的相似性,也存在遗传差异.这些结果表明应用RAPD技术可以很好地检测家兔不同品系间以及同一品系不同个体间的亲缘关系.

RAPD analysis of the genetic specificity of WHBE rabbit

Genetic relationship among White Hair and Black Eyes (WHBE) rabbits, Japanese big ear rabbits and New Zealand rabbits were analyzed with randomly amplified polymorphic DNA (RAPD) technique. Ten random primers were used to amplify genomic DNA of rabbits with PCR methods. On the basis of preliminary experiments, eight primers were selected to analyze the genetic variation among 3 rabbit lines. 107 amplified fragments were detected by electrophoresis on agarose gel. Among them, 32 fragments were polymorphic, accounting for 29.91% of the whole. It was showed that the RAPD polymorphisms among rabbit strain lines were abundant. The results of the statistical analysis of RAPD bands of rabbits showed that the genetic similarity between WHBE rabbits and Japanese big ear rabbit was 0.7948, which was the highest among 3 rabbit strain lines. Four primers among 10 random primers could not only amplify the common DNA bands of 3 rabbit strain lines, but also amplify the different characteristic bands of them. The amplified band patterns showed that there are both genetic similarities and genetic variations among WHBE rabbit, Japanese big ear rabbit and New Zealand rabbit. Thus, the RAPD technique could be used as a method to monitor genetic relationship among different strains and different individuals of the same strain of rabbits.