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耐热水稻基因型的鉴定及相关生理学特征分析(英文)
引用本文:周伟辉,薛大伟,张国平.耐热水稻基因型的鉴定及相关生理学特征分析(英文)[J].浙江大学学报(农业与生命科学版),2012(1):1-9.
作者姓名:周伟辉  薛大伟  张国平
作者单位:浙江大学农业与生物技术学院;杭州师范大学生命与环境科学学院
基金项目:Project supported by Zhejiang Provincial Science and Technology Bureau(2008C22069)
摘    要:对来自不同国家和地区的72个水稻品种在抽穗扬花期进行高温处理(40℃/35℃,14h/10h,12d),记录结实率,分析和耐热相关的生理学特征,并鉴定耐热水稻的基因型.2年的试验结果表明:高温对结实率有严重的伤害,且基因型间存在明显的差异;苗期高温处理显著降低了叶绿素含量(SPAD值)、株高、根长和生物量,且对敏感基因型的影响较耐热基因型大;同时,高温胁迫导致膜脂过氧化,表现为丙二醛含量增加和超氧化物歧化酶活性增强;与敏感基因型相比,耐热基因型的丙二醛含量增加较少,而超氧化物歧化酶活性增加较多;根据试验结果,春江06、密阳46和京系17为高耐热基因型,明恢63、Kasalath和IRAT4217为高热敏感基因型.

关 键 词:水稻  高温  活性氧  胁迫  耐热性

Identification and physiological characterization of thermo-tolerant rice genotypes
ZHOU Wei-hui,XUE Da-wei,ZHANG Guo-ping.Identification and physiological characterization of thermo-tolerant rice genotypes[J].Journal of Zhejiang University(Agriculture & Life Sciences),2012(1):1-9.
Authors:ZHOU Wei-hui  XUE Da-wei  ZHANG Guo-ping
Institution:1(1.College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China;2.College of Life and Environment Sciences,Hangzhou Normal University,Hangzhou 310036,China)
Abstract:The experiments were conducted to identify thermo-tolerant rice genotypes and characterize physiological traits relevant to heat stress tolerance.Seventy-two rice genotypes were exposed to high temperature(40 ℃/35 ℃,14 h/10 h) for 12 d at heading stage,and floret fertility was examined.The similar results were obtained in the two years’ experiments that there was a distinctly genotypic difference in floret fertility as affected by heat stress.When rice seedlings were exposed to heat stress,chlorophyll content(SPAD value),plant height,root length,shoot and root biomass were dramatically reduced,with thermo-tolerant genotypes being relatively less affected than sensitive ones.Meanwhile,high temperature enhanced membrane lipid peroxidation,as expressed by malondialdehyde content and increased superoxide dismutase(SOD) activity.Thermo-tolerant genotypes had less and more increase in MDA content and SOD activity than sensitive ones under heat stress relative to the control,respectively.Accordingly the thermo-tolerant genotypes were identified,with genotypes CJ6,MY46 and JX17 being highly thermo-tolerant,MH63,Kasalath and IRAT4217 being very sensitive.
Keywords:rice  high temperature  reactive oxygen species  stress  thermo-tolerance
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