猪BMP15和BMPR-IB基因的遗传变异及其与产仔性能的关系

采用PCR-SSCP方法对莱芜猪、鲁莱黑猪、里岔黑猪、鲁烟白猪、新沂蒙黑猪5个山东地方/培育猪种和大约克夏、长白、杜洛克3个引进猪种共8个猪种481头繁殖母猪进行BMP15和BMPR-IB基因的多态性分析,并采用最小二乘法分析其对产仔数的遗传效应.结果表明:2个基因的3个位点在8个猪种的测定群体中均存在多态性,但山东地方/培育猪种与引进猪种间在基因型频率上存在较大差异;BMP15和BMPR-IB基因对产仔数性状有显著影响(P0.05).对于BMP15基因,AA基因型母猪中,引进猪种比山东地方/培育猪种母猪的总产仔数和活产仔数平均多产1.20头和1.64头(P0.05);CC基因型母猪中,引进猪种比山东地方/培育猪种母猪的总产仔数和活产仔数平均多产1.20头和0.82头(P0.05).对于BMPR-IB基因,山东地方/培育猪种内AA基因型母猪的总产仔数和活产仔数比BB基因型母猪平均多产0.49头和0.51头(P0.05);引进猪种中BB基因型母猪比AA基因型母猪总产仔数和活产仔数平均多产1.05头和0.90头(P0.05).

Genetic variants of BMP15 and BMPR- IB genes and association with litter size in pig

Four hundred and eighty-one pigs including Laiwu Black,Lulai Black,Licha Black,Luyan White,New Yimeng Black,Yorkshire,Landrace and Duroc were selected to detect genetic variants of BMP15 and BMPR-IB genes by PCR-SSCP . The genetic effects of the two genes on litter size were analyzed by least squares method . The results showed that the polymorphic sites of both BMP15 and BMPR-IB genes were found in all populations tested . The genotype distribution,however,revealed great differences between Shandong local breeds and foreign pig breeds . The effects of genotypes on total number born (TNB) and number born alive (NBA) were significant ( P<0.0 5) . For BMP15 gene,in AA genotypic sows,the average TNB and NBA of foreign pig breeds were1 .20 and1.6 4 more than that of Shandong local breeds,respectively ( P<0.0 5);in CC genotypic sows,the average TNB and NBA of foreign pig breeds were 1.2 0 and 0.8 2 more than that of Shandong local breeds,respectively ( P < 0.0 5) . For BMPR-IB gene,in Shandong local breeds,the average TNB and NBA of AA were0.4 9 and 0.5 1 more than that of BB,respectively ( P>0.0 5);while the average TNB and NBA of BB was1.0 5 and0.9 0 more than that of AA in the foreign pig breeds,respectively ( P<0.0 5) .