| 壳聚糖对番茄枯萎病菌的拮抗作用 |
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| 引用本文： | 廖春燕,马国瑞,洪文英.壳聚糖对番茄枯萎病菌的拮抗作用[J].浙江大学学报(农业与生命科学版),2001,27(6):619-623. |
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| 作者姓名： | 廖春燕 马国瑞 洪文英 |
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| 作者单位： | 1. 浙江大学资源科学系,
2. 杭州市蔬菜研究所. |
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| 基金项目： | 浙江省科技厅资助项目(991103335) |
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| 摘 要： | 采用含毒介质法研究了壳聚糖对番茄枯萎病菌菌丝生长和孢子萌发的抑制作用.结果表明,浓度大于1 mg/mL的壳聚糖能明显抑制菌丝生长,各设定浓度均可在一定程度上抑制孢子萌发,而且抑制效果随着处理浓度的增大而增强;浓度大于0.1 mg/mL的壳聚糖处理可诱导初生菌丝发生肿胀、分枝增多且茵体分隔增加及体细胞变短等形态学变化.壳聚糖的抑菌作用机制与其增加茵丝细胞膜的透性有关.
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| 关 键 词： | 壳聚糖 番茄枯萎病菌 拮抗作用 |
| 文章编号： | 1008-9209(2001)06-0619-05 |
Inhibition of chitosan to the gowth of F. Oxysporum. F. Sp. Lycopersici |
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| LIAO Chun-yan ,MA Guo-rui ,HONG Wen-ying.Inhibition of chitosan to the gowth of F. Oxysporum. F. Sp. Lycopersici[J].Journal of Zhejiang University(Agriculture & Life Sciences),2001,27(6):619-623. |
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| Authors: | LIAO Chun-yan MA Guo-rui HONG Wen-ying |
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| Institution: | LIAO Chun-yan 1,MA Guo-rui 1,HONG Wen-ying 2 |
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| Abstract: | Chitosan was effective in inhibiting radial growth and sporogensis of F. oxysporum. f. sp. ylcopersici in vitro. The inhibiting activity of chitosan was increased when its concentration increased. Light microscope observations showed that chitosan at concentration greater than 0.1 mg/mL induced morphological changes, including hyphal swelling, distortion, excessive branching, shortening of hyphal segments in new hyphal of F. oxysporum. f. sp. lycopersici. The resistance mechanism of chitosan is related to its ability to increase the permeability of the fungal plasma membrane. |
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| Keywords: | chitosan F oxysporum f sp lycopersici resistance |
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