| 稻瘟病菌的拮抗细菌筛选 |
| |
| 引用本文： | 林福呈,徐,林维挺,陈卫良,李德葆.稻瘟病菌的拮抗细菌筛选[J].浙江大学学报(农业与生命科学版),1998(6). |
| |
| 作者姓名： | 林福呈 徐 林维挺 陈卫良 李德葆 |
| |
| 作者单位： | 浙江农业大学生物技术研究所 |
| |
| 摘 要： | 自稻叶及其它禾本科杂草叶片表面分离纯化９８５株细菌分离物，分别培养在ＮＢ液体培养基中，经８０００ｒ／ｍｉｎ离心１０ｍｉｎ后取上清液，加入稻瘟病菌分生孢子悬液中，测定对稻瘟病菌分生孢子发芽，附着胞形成的抑制作用，从而筛选出１３株对稻瘟病菌孢子芽管生长或附着胞形成具有抑制作用的拮抗细菌．采用离体稻叶接种测定，进一步筛选得到３株拮抗细菌，初步鉴定为：Ｐｓｅｕｄｏｍｏｎａｓｆｌｕｏｒｅｓｃｅｎｓ（Ｘｈ２１６），Ｂａｃｉｌｕｓｓｐ．（Ｘｈ２２２）及Ｐｓｅｕｄｏｍｏｎａｓｓｐ．（Ｘｈ２４０）．在离体条件下，Ｘｈ２１６，Ｘｈ２２２和Ｘｈ２４０对叶瘟的防治效果分别为７２．３％，７０．５％和６７．４％．
|
| 关 键 词： | 水稻 稻瘟病菌 拮抗细菌 |
Screening of antagonistic bacteria to Magnaporthe grisea . |
| |
| Lin Fucheng, Xu Hong, Lin Weiting, Chen Weiliang, Li Debao.Screening of antagonistic bacteria to Magnaporthe grisea .[J].Journal of Zhejiang University(Agriculture & Life Sciences),1998(6). |
| |
| Authors: | Lin Fucheng Xu Hong Lin Weiting Chen Weiliang Li Debao |
| |
| Abstract: | A total of 985 bacterial isolates were obtained from rice leaves and other grasses. The isolates were cultared in nutrient broth(NB) medium. After centrifugated, 8 000 r/min for 10 min, the supernatants of each bacterial NB cultures were tested for their suppressive effects on the germination and appressorium formation of Magnaporthe grisea . Of the tested isolates, 13 isolates showed suppressive effects. Three isolates identified as Psendomonas fluorescens (Xh216), Bacillus sp. (Xh222) and Pseudomonas . sp. (Xh240),suppressed the disease severities on the detached rice leaves with the disease severity reduction 72.3%, 70.5% and 67.4%, respectively. |
| |
| Keywords: | rice Magnaporthe grisea bacterial antagonists |
| 本文献已被 CNKI 等数据库收录！ |
|
