| 蛋白质标准曲线的横坐标单位为何可用蛋白质浓度 |
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| 引用本文： | 赵赣.蛋白质标准曲线的横坐标单位为何可用蛋白质浓度[J].宁夏农林科技,2011,52(4):33+36-33,36. |
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| 作者姓名： | 赵赣 |
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| 作者单位： | 华南农业大学生命科学院生物化学与分子生物学系,广东广州,510642 |
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| 摘 要： | 由于在试验条件下,蛋白质与考马斯亮蓝G-250结合形成的蛋白质-考马斯亮蓝G-250复合物的量取决于蛋白质的量,根据朗比尔定律的公式进行推导表明,虽然在试验中测定的是蛋白质-考马斯亮蓝G-250复合物溶液的光密度值,而所做蛋白质标准曲线的横坐标却可以蛋白质的浓度为单位。
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| 关 键 词： | 蛋白质含量 标准曲线 横坐标 蛋白质浓度 |
Why Could Protein Concentration Be Used Directly As the Unit of X-axis of Standard Curve for Protein Content Measuration |
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| ZHAO Gan.Why Could Protein Concentration Be Used Directly As the Unit of X-axis of Standard Curve for Protein Content Measuration[J].Ningxia Journal of Agriculture and Forestry Science and Technology,2011,52(4):33+36-33,36. |
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| Authors: | ZHAO Gan |
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| Institution: | ZHAO Gan(Department of Biochemistry and Molecular Biology,College of Life Science,South China Agricultural University,Guangzhou,Guangdong 510642) |
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| Abstract: | Under the experimental conditions,the amount of protein-Coomassie brilliant blue G-250 complex was decided by the one of protein,so it was deduced by Lambert-Beer law that protein concentration could be used directly as the unit of X-axis of standard curve for protein content measuration,although the optical density of the solution of protein-Coomassie brilliant blue G-250 complex was measured in the experiment. |
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| Keywords: | Content of protein Standard curve Abscissa axis Concentration of protein |
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