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L-亮氨酸产生菌的代谢工程改造及其发酵效率
引用本文:黄钦耿.L-亮氨酸产生菌的代谢工程改造及其发酵效率[J].台湾农业探索,2014(4):68-72.
作者姓名:黄钦耿
作者单位:[1]福建省麦丹生物集团有限公司福州研究中心,福建福州350008; [2]福建师范大学工业微生物发酵技术国家地方联合工程研究中心,福建福州350107
摘    要:以选育的获得产L-亮氨酸的谷氨酸棒杆菌MD106为出发菌株,通过重叠延伸PCR及自杀载体介导的同源重组技术构建panBC及alaT双基因缺失的突变株MD106ΔpanBC/ΔalaT,并对出发菌及双缺失重组菌进行摇瓶发酵试验,测定发酵指数。结果显示,发酵40h后,突变株的L-亮氨酸的产量为7.91g/L,比出发菌株提高43.3%,而且主要杂酸——丙氨酸减少超过80%,总杂酸比例较出发菌株减少55.6%。

关 键 词:L-亮氨酸  谷氨酸棒杆菌  代谢工程  杂酸

Metabolic Engineering Modifications of the L-leucine Producing Strain Corynebacteriurn glutamicum and Its Fermentation Efficiency
HUANG Qin-geng.Metabolic Engineering Modifications of the L-leucine Producing Strain Corynebacteriurn glutamicum and Its Fermentation Efficiency[J].Taiwan Agricultural Research,2014(4):68-72.
Authors:HUANG Qin-geng
Institution:HUANG Qin-geng (1. Fuzhou Research Center, Fujian Maidan Biological(Group) Co. Ltd. , Fuzhou, Fujian 350008, China; 2. National-Local United Engineering Research Center of Industrial Microorganism Fermentation Technology, Fujian Normal University, Fuzhou, Fujian 350107, China)
Abstract:Taking Corynebacterium glutamicurn MD106 as a starting strain applied to produce L-leucine, the mutant strain MD106△pan13C/AalaT from the double gene-negative of panBC and alaT was structured by using the methods of overlap extension PCR and suicide vector-mediated homologous recombination, and the fermentation index was measured through the ermentation test of the starting strain and the recombinant strain. The results showed that the L-leueine yield of the recombinant strain was 7.91g/L by Amino acid analyzer after40 h fermentation, and it increasing by 43.3 % comparing with that of the original strain MD106, and mainly miscellaneous acid--alanine reduced by more than 80%, the total ration of miscellaneous acid decreased 55.6% compared with the original strain.
Keywords:L-leucine  Corynebacterium glutamicum  metabolic engineering  miscellaneous acid
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