| 蝴蝶兰快速繁殖关键技术研究 |
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| 引用本文： | 刘彦珍,毛光志.蝴蝶兰快速繁殖关键技术研究[J].辽宁农业职业技术学院学报,2011,13(3):21-23. |
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| 作者姓名： | 刘彦珍 毛光志 |
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| 作者单位： | 安阳工学院生物与食品学院,河南安阳,455000 |
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| 摘 要： | 以蝴蝶兰嫩叶、茎尖、花梗侧芽、花梗节间为外植体,进行原球茎诱导,探求蝴蝶兰组织培养的最适外植体;通过不同培养基及各种激素配比组合进行原球茎诱导、增值及试管苗生根试验,探索各阶段最优培养基配方。结果表明：茎尖和花梗腋芽是蝴蝶兰形成原球茎的较佳外植体;MS＋0.5 mg/L NAA＋1.0 mg/L 6-BA＋100ml/L椰乳是蝴蝶兰原球茎增殖的最佳培养基,增殖系数达8.98;1/2 MS培养基最利于侧芽萌发形成丛生芽;生根最适培养基为1/2MS＋1.0mg/LIBA＋0.5 mg/LNAA。
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| 关 键 词： | 蝴蝶兰 快速繁殖 关键技术 |
Study on Key Technique of Rapid Propagation of Phalaenopsis amabilis |
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| LIU Yanzhen,MAO Guangzhi.Study on Key Technique of Rapid Propagation of Phalaenopsis amabilis[J].Journal of Liaoning Agricultural Vocation-Technical College,2011,13(3):21-23. |
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| Authors: | LIU Yanzhen MAO Guangzhi |
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| Institution: | LIU Yanzhen,MAO Guangzhi(Anyang Institute of Technology Biotechnology and Food Engineering,Anyang,455000,China) |
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| Abstract: | Picking tender leaves,shoot-tips,pedicel lateral bud,pedicel internode as explants to induce original corm,to search the best explants on tissue culturing of Phalaenopsis amabilis;Different culture medium with hormone combination were screened to original corm induction,proliferation and rooting of seedling in test tube;Searching the optimal medium in different culturing stage.The result showed:the shoot-tips and pedicel lateral buds are the best explants for inducing original corm;The best culture medium o... |
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| Keywords: | Phalaenopsis amabilis Fapid propagation keytechnology |
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