| 二种分离液对牛腔前卵泡卵母细胞体外培养效果观察 |
| |
| 引用本文： | 高建明,万善霞,高立云,郭勇,秦晓军.二种分离液对牛腔前卵泡卵母细胞体外培养效果观察[J].北京农学院学报,1999,14(4):38-41. |
| |
| 作者姓名： | 高建明 万善霞 高立云 郭勇 秦晓军 |
| |
| 作者单位： | 北京农学院动物科学系,北京,102206 |
| |
| 摘 要： | 本实验通过２种无血清分离液，采用机械方法分离牛腔前卵泡卵母细胞，体外培养７ｄ，观察其体外培养效果结果表明，Ｍ１９９Ｈｅｐｅｓ组腔前卵泡卵母细胞发育率为６３．４％，ＰＢＳ组为３０．３％，二者差异极显著（Ｐ＜０，０１）；第７ｄ 卵泡平均增长直径以Ｍ１９９Ｈ＿组好于ＰＢＳ组，分别为（２８． ７± ２１． ２）μ和（２１． ６± ８． ０）μｍ，次级卵泡（Ｓｃ）增长速率大于初级卵泡（Ｐｍ）
|
| 关 键 词： | 牛 腔前卵泡 体外培养 分离液 无血清 |
| 修稿时间： | 1999-07-19 |
Observing the Effect of Two Kinds of Isolation Mediums on In Vitro Culture of Bovine Preantral Follicles |
| |
| Gao Jianming.Observing the Effect of Two Kinds of Isolation Mediums on In Vitro Culture of Bovine Preantral Follicles[J].Journal of Beijing Agricultural College,1999,14(4):38-41. |
| |
| Authors: | Gao Jianming |
| |
| Abstract: | The isolation of preantral follicles from the ovaries of adult bovine was performedusing a simple, rapid mechanical method by two kinds of serum - free isolation mediums. Thepreantral follicles were cultured for 7d at 39 in 5%CO2 in air. The results showed that therewas significant difference between M199Hepes isolation medium and PBS isolation medium indevelopmental rates (63. 4 % vs 30. 3 % ) (P< 0. 01 ). M199Hepes group was better than PBSgroup in the mean follicular increase at Day 7 of culture, (28. 7 21. 2 ) m and(21. 6 8. 0)m,respectively. The increase rates of follicular diameter were different betweensecondary follicles and primary follicles. |
| |
| Keywords: | Bovine preantral follicles in vitro culture isolation medium serum - free |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
