HPLC法检测功能红曲中的洛伐他汀

采用高效液相色谱法 (HPLC) ,选用SpherisorbODS柱 ,以甲醇 0 1%磷酸 (75∶2 5)分离测定功能红曲中的洛伐他汀 ,检测波长 2 37nm .该方法具有良好的线性关系和分离度 ,重现性好 ,平均回收率为 10 0 43% ,RSD为 1 82 % .本文对功能红曲样品进行测定 ,最高洛伐他汀含量为 2 38 2 1μg/ g .此法快速、准确 ,可作为此类产品质量控制方法

Determination of Lovastatin in Functional Red Koji Using HPLC Method

The paper describes the determination of Lovastatin in functional red koji by means of HPLC(high performance liquid chromatoguphic). Lovastatin is separated on the Spherisorb ODS column using methanol 0 1% phosphoric acid (75∶25) as mobile phase. Detection wavelength is 237 nm. The method demonstrates good linear relationship, separation drgree and reproducibility. The average recovery rate of Lovastatin is 100 43% and RSD (Relative Standard Deviation) is 1 82%. The experiment shows that the highest content of Lovastatin in functional red koji is 238 21μg/g. The approach has characteristics of quickness and accuracy, which can serve as the quality control method of the similar products.