| ‘红颜’草莓茎尖培养与快速繁殖 |
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| 引用本文： | 晁慧娟,刘敏,姬谦龙,高遐虹,董清华.‘红颜’草莓茎尖培养与快速繁殖[J].北京农学院学报,2009,24(4):14-16. |
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| 作者姓名： | 晁慧娟 刘敏 姬谦龙 高遐虹 董清华 |
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| 作者单位： | 1. 北京农学院植物科学技术学院,北京,102206
2. 北京农学院组培实验中心,北京,102206 |
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| 基金项目： | 北京市教委科技发展计划项目，昌平区科委科技发展计划项目 |
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| 摘 要： | 对‘红颜’草莓茎尖进行组织培养研究，建立其再生快繁体系。结果表明：（1）剥取的茎尖越大，萌发率越高；（2）暗培养3d能够抑制褐化，提高萌发率；（3）增殖培养基：MS＋6-BA0．5mg／L＋NAA0．10mg／L，增殖系数为7．00；（4）生根培养基：1／2MS＋IBA0．5mg／L，生根率为100％，移栽到消毒过的蛭石＋草炭＋珍珠岩（1：1：1），成活率为100％。
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| 关 键 词： | 红颜品种 草莓 茎尖培养 快速繁殖 |
Stem Tip Culture and Rapid Propagation of 'Benihoppe' Strawberry |
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| CHAO Hui-juan,LIU Min,JI Qian-long,GAO Xia-hong,DONG Qing-hua.Stem Tip Culture and Rapid Propagation of ''Benihoppe'' Strawberry[J].Journal of Beijing Agricultural College,2009,24(4):14-16. |
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| Authors: | CHAO Hui-juan LIU Min JI Qian-long GAO Xia-hong DONG Qing-hua |
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| Institution: | CHAO Hui-juana,LIU Mina,JI Qian-longb,GAO Xia-honga,DONG Qing-huaa(a.Plant Science , Technology College,b.Center of Tissue Culture,Beijing University of Agriculture,Beijing 102206,China) |
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| Abstract: | The system of rapid propagation was established by tissue culture of stem tip ofˋbenihoppeˊstrawberry.The results showed that the best media or treatment for various stages were as follows:(1)The larger the stem tips had the higher the survival rate;(2)Darkness treatment for 3 d could inhibited browning rate and increased survival rate;(3)The proliferation coefficient was 7.00 in medium MS+6-BA0.5mg/L +NAA 0.10 mg/L;(4)The rooting rate reached to 100% in medium 1/2 MS+IBA0.5 mg/L,and surviving rate was up to 100% when transplanted to the disinfected composing soil(vermiculite:turf:perlite,1∶1∶1). |
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| Keywords: | Benihoppe variety strawberry stem tip culture rapid propagation |
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