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鸡柔嫩艾美耳球虫单卵囊分离技术的建立
引用本文:安健,汪明,王黎霞,郝霖雨,郭睿,徐建华.鸡柔嫩艾美耳球虫单卵囊分离技术的建立[J].北京农学院学报,2004,19(1):5-6,13.
作者姓名:安健  汪明  王黎霞  郝霖雨  郭睿  徐建华
作者单位:1. 北京农学院动物科学技术系,北京,102206
2. 中国农业大学动物医学院,北京,100094
3. 山东青州农业综合开发办公室,山东,262600
基金项目:国家科技攻关项目 , 北京市自然科学基金
摘    要:以玻璃纸做材料,用单卵囊分离技术,对柔嫩艾美耳球虫进行了分离,实验室单卵囊感染26只鸡,在感染后5~12d,用饱和盐水漂浮集卵法进行检测,其中10只鸡检出卵囊,结果表明,该单卵囊分离技术简单易行,单卵囊感染成功率达38%,单卵囊技术的关键步骤为:(1)滴管拉的尽量细,(2)滴液尽量小,(3)要耐心观察,(4)实验鸡在0~7日龄。

关 键 词:柔嫩艾美耳球虫  单卵囊分离技术  
文章编号:1002-3186(2004)01-0005-02

Single Oocyst Techniqual Isolation of Eimeria tenella
AN Jian,WANG Ming.Single Oocyst Techniqual Isolation of Eimeria tenella[J].Journal of Beijing Agricultural College,2004,19(1):5-6,13.
Authors:AN Jian  WANG Ming
Institution:AN Jian~1,WANG Ming~2
Abstract:E. tenella is isolated by single oocyst technique with plastic paper. The main steps consist of: (1) pulling the end of the pipe as thin as possible. (2) dropping of oocyst solution as small as possible.(3) observing with patience when the experiment is done. (4) The age of chickens should be among 1 to 7 days. Each of 26 chickens is inoculated single oocyst with plastic paper. The oocyst in farces of chickens is identified by saturated sodium chloride solution floating. The oocyst is found in 10 of 26 chickens. The isolated rate is 38 percent.
Keywords:Eimeria tenella  single oocyst technique  chicken
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