植物样品中游离氨基酸总量测定方法的改进

本文根据氨基酸与茚三酮显色反应的原理，对传统测定植物鲜样游离氨基酸总量的方法作了改进。用沸水浴处理植物样品提取液可以破坏样品中抗坏血酸，使得样品提取液与制作氨基酸标准曲线反应液的抗坏血酸完全控制在同一水平上，避免了样品与标准曲线反应液由于抗坏血酸含量不同造成的误差。另外适当提高反应体系抗坏血酸的浓度可以提高反应灵敏度。

Path Analysis of Individual Plant Productivity ofFresh Ears for Ceratina Kulesh

Abstract The traits with individual plant productivity of Cerativa kulesh were investigated using 36 cross combinations of Cerativa kulesh. Based on Correlation analysis of simple, phenotype and genotype analysis, Path diaqram of the individual plant productivity of the fresh ears were estab- lished by the trait correlation and causality. The results showed that numbers of kerners each spike, rate of proclucing kernel, number of kernels each line, ear length, spike size and develoep- ment periods increased were signififcaut to improved the individual plant producfivity of the freshears; the traits such as the kernel weigh/100, plants number/unit area were negative effeet on the individual plant productivity. Direct effect and indirect effect of the traits on the productivity were analysed, which provides reference and bases in inbreeding line development , parent selection , se- lection efficiency increased.