牛输卵管蛋白对小鼠原核胚体外发育的影响

本实验通过添加１～４ｍｇ／ｍｌ牛输卵管蛋白至ＴＣＭ－１９９培养液中，对小鼠原核胚进行体外培养，观察原核胚的分裂和发育情况。小鼠原核胚很少分裂，远低于对照组原核胚分裂和发育水平。牛输卵管蛋白对小鼠原核胚的发育起明显的抑制作用，同时这种抑制作用随牛输卵管蛋白浓度的降低．似乎有减轻的趋势，表明牛输卵管蛋白中，存在抑制小鼠胚胎分裂和发育的蛋白质，对小鼠原核胚的抑制作用具有剂量效应。

The Influence of Bovine Oviducal Fluid Proteins(BOF)on the Development of Pronuclear Mouse Embryos in Vitro

Bovine oviducts were Harvested fron local slaughterhouse at either the follicular orluteal stage of the oestruse cycle of the killed cattles. Nevertheless,bovine oviducal fluicl proteins(BOF),collected by flushing the oviduct lumen with normal saline,were attained by lyophilizigthe dialyzed flushings. BOF used for pronuclear monse embryos culture, the experiment was con-ducted to investigate in vitro development of one-cell mouse embryos in TCM-199 + 25mMHepessupplemented with BOF at the concentrations of 1～4mg/ml.Cultured in TCM-199 + BOF,cleavage to 2.cell or 3,4-cell stages hardly happened in comparison to the control treatment.Thisstudy indicated that additlon of BOF to TCM-199 retarded embryo development in vitro comparedto controls,But as if BOF effects on embryo Cleavage Was dose-dependent,A reduction of thepresence of BOF in the TCM-199 resulted in embyos cleaving ( 1mg/ml BO),it was suggestedthat an inhibitor of embryonic development was present in BOF, and its unproductive or detri-mental offect on development of 1-cell mouse embryos decreased when BOF dilnteel in TCM-199to the conentration of 1mg/ml.