奶牛乳腺上皮细胞的原代培养

【目的】探索一种简单且高效的原代奶牛乳腺上皮细胞的培养方法。【方法】采用组织块培养法、酶消化法培养奶牛乳腺上皮细胞;通过MTT法检测吸光度绘制细胞生长曲线;通过免疫组化法鉴定角蛋白-18的表达。【结果】酶消化法相比组织块培养法获得的细胞具有耗时短,获得量高等优点,MTT法绘制的生长曲线符合一般生物学特性,呈"S"型,奶牛乳腺上皮细胞中角蛋白-18阳性表达。【结论】酶消化法是一种简单且高效的培养原代奶牛乳腺上皮细胞的方法。

Primary culture of bovine mammary epithelial cells

Objective]The objective of this study was to investigate a simple and efficient method for bovine mammary epithelial cells culture in vitro.Methods]Bovine mammary epithelial cells were cultivated by tissue explants cultivation and enzyme digestion methods,respectively.The absorbance was measured by MTT to draw the cell growth curves.The cytokeratin-18 expression was identified by immunohistochemistry.Results]The cell growth curve showed a typical "S" shape,in line with the general biological characteristics.Compared with tissue explants,enzyme digestion method has the advantage of shorter time-consumption and higher production and so on.The cytokeratin-18 was detected positive by immunohistochemistry.Conclusion] Enzyme digestion method is a simple and efficient way to culture bovine mammary epithelial cells.