| 南方根结线虫在不同茉莉酸含量番茄株系中发育状态观察 |
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| 引用本文： | 李小曼,刘杨,刘洁,王绍辉,赵文超.南方根结线虫在不同茉莉酸含量番茄株系中发育状态观察[J].北京农学院学报,2017,32(4). |
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| 作者姓名： | 李小曼 刘杨 刘洁 王绍辉 赵文超 |
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| 作者单位： | 北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206 |
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| 基金项目： | 北京市自然科学基金项目，北京创新团队现代农业产业技术体系 |
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| 摘 要： | 【目的】探究内源茉莉酸对番茄中南方根结线虫生长发育的影响。【方法】以番茄野生型株系CM、过量表达前系统素转基因番茄35S∷PS(35S∷prosystemin)和茉莉酸合成缺失突变体spr2为试验材料,采用石蜡切片的方法,观察接种南方根结线虫二龄幼虫(J2)后不同时间各株系内线虫的发育。【结果】接种南方根结线虫J2 5d和7d,CM、35S∷PS和spr2根系中线虫发育无显著差异;接种J2 14d,21d和28d,35S∷PS根系中线虫发育较CM根系中线虫和spr2根系中线虫缓慢。【结论】茉莉酸在一定程度上可以抑制南方根结线虫在番茄中的发育。
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| 关 键 词： | 番茄 茉莉酸 根结线虫 发育状态 |
Observation of developmental status of Meloidogyne incongnita in tomato plants with different jasmonic acid content |
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| LI Xiaoman,LIU Yang,LIU Jie,WANG Shaohui,ZHAO Wenchao.Observation of developmental status of Meloidogyne incongnita in tomato plants with different jasmonic acid content[J].Journal of Beijing Agricultural College,2017,32(4). |
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| Authors: | LI Xiaoman LIU Yang LIU Jie WANG Shaohui ZHAO Wenchao |
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| Abstract: | objective]To investigate the effects of jasmonic acid on the growth and development of root knot nematodes in toma to.Methods]Tomato CM (wide tipe),JA overexpressed transgenic tomato line 35S::prosystemin (35S ∷ PS) and JA biosynthetic mutant (spr2) were used to inoculate RKN J2.The development status of nematodes in CM,35S ∷ PS and spr2 was observed by paraffin sections.Results]After inoculation RKN J2 5 and 7 d,we found there were no significant differences in nematode development status among CM,35S::PS and spr2,and after inoculation RKN J2 14,21 and 28 d,the nematodes development in the root of 35S::PS were slower than that in CM roots and spr2 roots.Conclusion]Jasmonic acid could inhibit the development of root knot nematode in tomato to some extent. |
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| Keywords: | tomato jasmonic acid root-knot nematode development status |
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