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一串红株型突变体AFLP分析
引用本文:杨建玉,陈洪伟,刘克锋,王红利,金珠理达,张睿.一串红株型突变体AFLP分析[J].北京农学院学报,2010,25(2):1-4.
作者姓名:杨建玉  陈洪伟  刘克锋  王红利  金珠理达  张睿
作者单位:北京农学院园林学院,北京,102206;北京农学院城乡发展学院,北京,102206
基金项目:北京市教育委员会项目 
摘    要:一串红(Salvia splendens)株型突变体BN35—1分枝能力强,不必摘心,株型自然成球。本研究利用AFLP(amplified fragment lengthpolymorphism)分子标记技术对其进行分析。从21对引物组合中筛选出6对多态性高的引物组合对突变体BN35—1、野生型BN35及4个商品种进行亲缘关系分析以确定BN35—1与BN35亲缘关系。另外用21对供试引物组合单独对突变体及原种进行多态性分析以寻找差异条带。结果表明,BN35—1与BN35亲缘关系最近,相似系数为0.9861,远高于其他商品种、BN35与商品种以及商品种间的相似性。在21对供试引物组合中,12对引物检测出BN35—1与BN35基因组DNA共52处基因座位位点存在差异,相应的特异条带是控制株型突变或与控制株型突变基因相连锁的侯选基因片段,深入研究工作有待进一步进行。

关 键 词:一串红  株型突变体  AFLP分子标记  多态性  亲缘关系

AFLP Analysis on the Plant-type Mutants of Salvia splendens
YANG Jian-yu,CHEN Hong-wei,LIU Ke-feng,WANG Hong-li,JIN Zhu-li-da,ZHANG Rui.AFLP Analysis on the Plant-type Mutants of Salvia splendens[J].Journal of Beijing Agricultural College,2010,25(2):1-4.
Authors:YANG Jian-yu  CHEN Hong-wei  LIU Ke-feng  WANG Hong-li  JIN Zhu-li-da  ZHANG Rui
Institution:YANG Jian-yua,CHEN Hong-weib,LIU Ke-fengb,WANG Hong-lia,JIN Zhu-li-daa,ZHANG Ruia(a.Landscape Design & Forestry College,b.Urban and Rural Development College,Beijing University of Agriculture,Beijing 102206,China)
Abstract:Plant-type mutants BN35-1 of Salvia splendens had high ramifications.They became ball-plant type without disbudding until maturate.The AFLP(amplified fragment length polymorphism) analysis was conducted in the study.To analyze relationship between BN35-1 and BN35,6 pairs of primers combination were selected from 21 pairs of primers combination to amplify plant-type mutants BN35-1,wild-tupe strain BN35 and 4 cultivars of S.splendens.Then 21 pairs of primers combination were used to amplify different band pat...
Keywords:Salvia splendens  Plant-type Mutant  AFLP Molecular Marker  Polymorphism  Genetic relationship  
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