| 苦参碱对小菜蛾幼虫毒力测定及其对害虫体内2种酶活性的影响 |
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| 引用本文： | 孟琳琳,王进忠,孙淑玲,张志勇,张民照,张鹏飞,丁佳,蒲媛媛.苦参碱对小菜蛾幼虫毒力测定及其对害虫体内2种酶活性的影响[J].北京农学院学报,2010,25(2):29-32. |
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| 作者姓名： | 孟琳琳 王进忠 孙淑玲 张志勇 张民照 张鹏飞 丁佳 蒲媛媛 |
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| 作者单位： | 北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206 |
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| 基金项目： | 北京市教育委员会项目，北京市自然科学基金，北京市科委资助项目，北京市市管高校人才强教计划资助项目，国家自然科学基金 |
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| 摘 要： | 采用生物测定和酶活力测定方法，研究苦参碱对小菜蛾Plutella xylostella L．的胃毒活性及其对幼虫体内谷胱甘肽-S-转移酶、乙酰胆碱酯酶活性的影响。试验结果表明：苦参碱对小菜蛾幼虫具有一定的胃毒作用。2mg／mL苦参碱处理叶片饲喂小菜蛾幼虫12h校正死亡率为37．93％，72h后小菜蛾幼虫的校正死亡率为95．83％。不同质量浓度苦参碱处理小菜蛾幼虫后，幼虫体内的谷胱甘肽-S-转移酶（GSTs）和乙酰胆碱酯酶（AchE）活力与对照相比均产生波动。经125×10 -3mg／mL苦参碱处理4h，小菜蛾体内GSTs活力最高，为1610．72U／（mg·min）。经125×10mg／mL苦参碱处理16h，小菜蛾体内的AChE酶活力最高，为10．63U／（mg·min）。各处理24h，小菜蛾体内GSTs活力和AChE酶活力均有明显降低。说明苦参碱对小菜蛾幼虫体内谷胱甘肽-S-转移酶活力和乙酰胆碱酯酶活力有一定的影响。
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| 关 键 词： | 苦参碱 小菜蛾 谷胱甘肽-S-转移酶 乙酰胆碱酯酶 |
Bioassay of Matrine and Its Effect on Two Enzyme Activities of Plutella xylostella Larvae |
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| MENG Lin-lin,WANG Jin-zhong,SUN Shu-ling,ZHANG Zhi-yong,ZHANG Min-zhao,ZHANG Peng-fei,DING Jia,PU Yuan-yuan.Bioassay of Matrine and Its Effect on Two Enzyme Activities of Plutella xylostella Larvae[J].Journal of Beijing Agricultural College,2010,25(2):29-32. |
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| Authors: | MENG Lin-lin WANG Jin-zhong SUN Shu-ling ZHANG Zhi-yong ZHANG Min-zhao ZHANG Peng-fei DING Jia PU Yuan-yuan |
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| Institution: | MENG Lin-lin,WANG Jin-zhong,SUN Shu-ling,ZHANG Zhi-yong,ZHANG Min-zhao,ZHANG Peng-fei,DING Jia,PU Yuan-yuan(Plant Science and Technology College,Beijing University of Agriculture,Beijing 102206,China) |
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| Abstract: | Stomach toxicity of matrine to 3rdlarvae of Plutella xylostella and its effects on activities of glutathione-S-transferase(GSTs)and acetylesterase(AChE) were tested with bioassay and emzyme activity determination.The results showed that matrine had the stomach poisoning action on the Plutella xylostella larvae.At the concentration of 2mg/mL,the corrected mortality of the larvae was 37.93% at 12h after matrine application,while the corrected mortality of the larvae was 95.83% at 72 h.The GSTs and AChE activi... |
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| Keywords: | matrine Plutella ylostella L glutathione-S-transferase(GSTs) acetylesterase(AChE) |
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